| 农杆菌介导的杜氏盐藻Dscbr基因转化紫花苜蓿的初步研究 |
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| 引用本文： | 陈晨,乔代蓉,白林含,马梵辛,贺顺姬,曹毅.农杆菌介导的杜氏盐藻Dscbr基因转化紫花苜蓿的初步研究[J].四川大学学报(自然科学版),2005,42(3):567-570. |
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| 作者姓名： | 陈晨 乔代蓉 白林含 马梵辛 贺顺姬 曹毅 |
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| 作者单位： | 四川大学生命科学学院,成都,610065;四川大学生命科学学院,成都,610065;四川大学生命科学学院,成都,610065;四川大学生命科学学院,成都,610065;四川大学生命科学学院,成都,610065;四川大学生命科学学院,成都,610065 |
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| 基金项目： | 国家高技术863项目(2001AA212161) |
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| 摘 要： | 利用农杆菌介导法进行紫花苜蓿遗传转化研究．将从盐生杜氏藻(Dunaliella samlina)中克隆到的抗逆基因Dscbr(编码一种早期光诱导蛋白)导入紫花苜蓿栽培种“中苜一号”，获得52个转化株系．经过卡那霉素抗性鉴定和PCR分子检测，获得6株阳性苗，PCR阳性率为11．54％．结果表明Dscbr基因已整合到苜蓿的基因组中．
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| 关 键 词： | 紫花苜蓿 Dscbr基因 转基因植株 根癌农杆菌 |
| 文章编号： | 0490-6756(2005)03-0567-04 |
Transformation of Alfalfa (Medicago sativa L. ) by Agrobacterium-mediated Process with Dscbr Genefrom Dunaliella salina |
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| CHEN Chen,QIAO Dai-rong,BAI Lin-han,MA Fan-xin,HE Shun-ji,CAO Yi.Transformation of Alfalfa (Medicago sativa L. ) by Agrobacterium-mediated Process with Dscbr Genefrom Dunaliella salina[J].Journal of Sichuan University (Natural Science Edition),2005,42(3):567-570. |
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| Authors: | CHEN Chen QIAO Dai-rong BAI Lin-han MA Fan-xin HE Shun-ji CAO Yi |
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| Abstract: | Alfalfa cultivar ZhongMuYiHao was transformed with Dscbr gene from Dunaliella Salina via Agrobacterium-mediated process. 52 regenerated alfalfa plants were obtained . Kanamycin resistance and PCR amplification analysis showed that 6 putative transgenic plants had the Dscbr gene, and the transformation ratio was 11.54 percent. The stress resistance of putative transgenic plants in laboratory and in field will be tested. |
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| Keywords: | alfalfa Dscbr gene transgenic plants Agrobacterium tumefactions |
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