| 变构菌素产生菌 S.griseochromogenes 接合转移体系建立与优化 |
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| 引用本文： | 王芬,邱荣国,唐莉.变构菌素产生菌 S.griseochromogenes 接合转移体系建立与优化[J].大连理工大学学报,2012,52(3):338-342. |
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| 作者姓名： | 王芬 邱荣国 唐莉 |
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| 作者单位： | 大连理工大学分子药物研究中心,辽宁大连,116024 |
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| 基金项目： | 国家杰出青年科学基金资助项目 |
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| 摘 要： | 以整合型质粒pSET152为出发质粒,建立并优化了变构菌素产生菌Streptomyces griseochromogenes(S.griseochromogenes)与Escherichia coli(E.coli)的属间接合转移体系,确定了适用于变构菌素产生菌灰产色链霉菌的最佳接合转移条件.研究发现Ca2+和甘氨酸都能够提高接合转移的效率,尤其是甘氨酸的效果非常显著.这一工作对于S.griseochromogenes的基因操作、小分子药物生物合成机制的研究以及具有自主知识产权的药物衍生物的开发具有普遍的应用价值.
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| 关 键 词： | Streptomyces griseochromogenes 接合转移 甘氨酸 钙离子 |
Establishment and optimization for conjugal transfer system of tautomycetin-producing strain S.griseochromogenes |
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| WANG Fen,QIU Rongguo,TANG Li.Establishment and optimization for conjugal transfer system of tautomycetin-producing strain S.griseochromogenes[J].Journal of Dalian University of Technology,2012,52(3):338-342. |
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| Authors: | WANG Fen QIU Rongguo TANG Li |
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| Institution: | (Research Center for Molecular Medicine,Dalian University of Technology,Dalian 116024,China) |
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| Abstract: | Intergeneric conjugal transfer system from Escherichia coli(E.coli) to tautomycetin-producing strain Streptomyces griseochromogenes(S.griseochromogenes) is established and optimized,which uses integrative plasmid pSET152 as starting plasmid.The best conjugal transfer conditions for S.griseochromogenes are demonstrated.And it is found that Ca2+ and especially glycine,can increase the efficiency of exconjugates.The experimental results are very helpful to genetic manipulation of S.griseochromogenes,bio-synthesized mechanism research for small molecule drugs and for developing new analogs of drug with independent knowledge property. |
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| Keywords: | Streptomyces griseochromogenes conjugal transfer glycine Ca2+ |
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