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| 改良碱变性法抽提冷冻山羊肝脏组织线粒体DNA | |
| 引用本文： | 李力,姚绍嫦,孙群,赵建,张杰,杨志荣.改良碱变性法抽提冷冻山羊肝脏组织线粒体DNA[J].四川大学学报(自然科学版),2007,44(6):1331-1334. |
| 作者姓名： | 李力 姚绍嫦 孙群 赵建 张杰 杨志荣 |
| 作者单位： | 1. 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610064;广西药用植物园,南宁,530023 2. 广西药用植物园,南宁,530023 3. 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610064 |
| 摘 要： | 通过实验建立了一种简便、快捷地从冷冻山羊肝脏组织中制备高质量线粒体DNA（mtDNA）的方法.以差速离心法分离线粒体，用碱性SDS法裂解线粒体膜，释放线粒体DNA后用酚／氯仿抽提.与其他方法相比较，该方法具有对组织新鲜度要求不高，快速、简便、经济、产品产量高以及稳定性好等特点，其得率和纯度均能满足mtDNA多样性分析的要求. |
| 关 键 词： | 动物 改良碱变性法 |
| 文章编号： | 0490-6756（2007）06-1331-04 |
| 收稿时间： | 2007-05-24 |
| 修稿时间： | 2007-11-07 |
Improved alkali lysis method for mitochondrial DNA preparation from frozen goat liver |
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| LI Li,YAO Shao-chang,SUN Qun,ZHAO Jian,ZHANG Jie and YANG Zhi-rong.Improved alkali lysis method for mitochondrial DNA preparation from frozen goat liver[J].Journal of Sichuan University (Natural Science Edition),2007,44(6):1331-1334. | |
| Authors: | LI Li YAO Shao-chang SUN Qun ZHAO Jian ZHANG Jie and YANG Zhi-rong |
| Institution: | Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University;Institute of Guangxi Medicinal Plant;Institute of Guangxi Medicinal Plant;Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University;Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University;Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University;Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Science, Sichuan University |
| Abstract: | A simple and rapid method was developed to isolate high quality mitchondrial DNA (mtDNA) from frozen goat liver. Mitochondria were isolated by velocity-gradient centrifugation and the mitochondrion membrane was broken up by alkali lysis. mtDNA was then extracted by phenol and chloroform. Compared with other methods, this method has less strict requirement on the tissue freshness. It is also simple, rapid and economic for preparing mtDNAs with both high product and high stability. Both the recovery rate and purity of the isolated mitchondrial DNA with this method can meet the need of mtDNA diversity analysis. |
| Keywords: | mtDNA |
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