| 正交试验法确定烟草甲成虫羧酸酯酶反应的最适条件 |
| |
| 引用本文： | 柳琼友,陈文龙,顾丁,高光澜.正交试验法确定烟草甲成虫羧酸酯酶反应的最适条件[J].西南师范大学学报(自然科学版),2011,36(3). |
| |
| 作者姓名： | 柳琼友 陈文龙 顾丁 高光澜 |
| |
| 作者单位： | 贵州大学昆虫研究所,贵州省山地农业病虫害重点实验室,贵阳550025 |
| |
| 基金项目： | 中国烟草总公司贵州省公司科技资助项目，贵州大学引进人才科研资助项目 |
| |
| 摘 要： | 应用正交表L25(56)设计正交试验,研究了羧酸酯酶质量浓度、体积分数浓度、反应体系的pH值、反应温度和反应时间等5个因素对烟草甲成虫羧酸酯酶活性检测的影响,并从试验组合中选出最佳条件.在进行极差分析、方差分析和多重比较后,选择各因素的最优水平,得到了烟草甲成虫羧酸酯酶活性测定的最适条件,即酶的质量浓度为1头/mL、底物的体积分数为5.0×10-4 mo1/L,pH值为7.5,温度为42℃,时间为10 min.
|
| 关 键 词： | 烟草甲 羧酸酯酶 正交试验 方差分析 最佳条件 |
The Optimization of Conditions for Assaying Activity of Carboxylesterase from the Adults of Cigarette Beetle Lasioderma serricorne (Fabrcius) by Orthogonal Matrix Method |
| |
| LIU Qiong-you,CHEN Wen-long,GU Ding,GAO guang-lan.The Optimization of Conditions for Assaying Activity of Carboxylesterase from the Adults of Cigarette Beetle Lasioderma serricorne (Fabrcius) by Orthogonal Matrix Method[J].Journal of Southwest China Normal University(Natural Science),2011,36(3). |
| |
| Authors: | LIU Qiong-you CHEN Wen-long GU Ding GAO guang-lan |
| |
| Institution: | LIU Qiong-you,CHEN Wen-long,GU Ding,GAO guang-lan Institute of Entomology of Guizhou University,Guizhou Key Laboratory for Plant Pest Management of Mountainous Region,Guiyang 550025,China |
| |
| Abstract: | The affects of CarE concentration,substrate a-Naphthyl acetate(a-NA) concentration,pH of the reaction system,the reaction temperature and the reaction time on assaying of the specific activity of CarE in the adults of Lasioderma serricorne(Fabrcius) were studied by orthogonal matrix methods.L25(56) orthogonal matrix was adopted without considering interaction.The optimal conditions for assaying the activity of CarE in the adult beetles,through range analysis,analysis of variance and multiple comparison,were... |
| |
| Keywords: | Lasioderma serricorne(Fabrcius) carboxylesterase orthogonal experiment variance analysis optimal conditions |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《西南师范大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《西南师范大学学报(自然科学版)》下载免费的PDF全文 |
|
