| H3N2SIV河南株非结构蛋白1(NS1)重组的纯化及Dot-ELISA的建立 |
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| 引用本文： | 赵朴,郑玉姝,贾贝贝,赵坤,姚四新,王三虎,刘兴友,刘丽艳.H3N2SIV河南株非结构蛋白1(NS1)重组的纯化及Dot-ELISA的建立[J].西南师范大学学报(自然科学版),2011,36(3). |
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| 作者姓名： | 赵朴 郑玉姝 贾贝贝 赵坤 姚四新 王三虎 刘兴友 刘丽艳 |
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| 作者单位： | 河南科技学院动物科学学院,河南新乡453003;动物疫病和残留物防控河南省高校工程技术研究中心,河南新乡453003 |
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| 基金项目： | 河南科技学院青年基金资助项目 |
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| 摘 要： | 为建立鉴别猪流感病毒感染猪和灭活疫苗免疫猪的诊断方法,利用pET-28a载体在大肠杆菌BL21中表达H3N2 SIV河南株NS1,并通过Ni2+-NTA亲和色谱法纯化His-NS1融合蛋白.用SDS-PAGE,Western blotting和Dot-ELISA分析纯化蛋白,表明重组NS1能与猪流感病毒感染猪血清进行特异性反应,而不与灭活疫苗免疫猪血清反应.结果表明重组蛋白能作为区别猪流感病毒感染猪和灭活疫苗免疫猪的诊断抗原,为控制猪群中的流感病毒奠定了基础.
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| 关 键 词： | 猪流感病毒 非结构蛋白1 纯化 鉴别诊断 |
Purification of the Recombinant NS1 Protein of H3N2 SIV in He'nan Province and Preliminary Establishment of a Dot-ELISA |
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| ZHAO Pu,ZHENG Yu-shu,JIA Bei-bei,ZHAO Kun,YAO Si-xin,WANG San-hu,LIU Xing-you,LIU Li-yan.Purification of the Recombinant NS1 Protein of H3N2 SIV in He'nan Province and Preliminary Establishment of a Dot-ELISA[J].Journal of Southwest China Normal University(Natural Science),2011,36(3). |
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| Authors: | ZHAO Pu ZHENG Yu-shu JIA Bei-bei ZHAO Kun YAO Si-xin WANG San-hu LIU Xing-you LIU Li-yan |
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| Abstract: | To develope a diagnostic method of differentiating the infected from the vaccinated animals,NS1 of Swine influenza virus(SIV)isolated in Henan was expressed in BL21(pET-28a),and His-NS1 fusion protein was purified by Ni2+-NTA affinity chromatography.The purified NS1 protein was analyzed by SDS-PAGE,Western blotting and dot-ELISA.Results showed that the recombinant NS1 protein can react specifically with sera from pig infected with SIV,but not sera from pig immuned with inactived vaccine.It is concluded that... |
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| Keywords: | swine influenza virus nonstructura1 protein 1 purification differential diagnosis |
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