芦荟的组织培养和快速繁殖

以芦荟（Aloe）为试验材料,进行组培快繁实验,研究了激素对不定芽诱导、试管苗繁殖、试管苗生根等的影响,同时对不同栽培基质进行了筛选。根据研究结果,指出对芦荟不定芽诱导效果最佳,对试管苗增殖率最高的培养基一致,均为MS＋6-BA4mg/L＋NAA0.5mg/L（pH5.8～6.0,食糖3%,琼脂0.8%）,芦荟试管苗月增殖率为19.60倍,年增殖率可达6400万倍,在芦荟生根培养中,1/2MS＋IBA1.5mg/L＋活性炭0.3g/L＋蔗糖30g/L＋琼脂7g/L的培养基较好,最佳栽培基质为河沙。

Tissue Culture and Rapid PrOpagation of Aloe

Taking Aloe as the test material, this paper carries out the test on the tissue culture and rapid propagation, researches the influences of hormones on the inducement of adventitious bud, tube seedling propagation and tube seeding rooting, and filters the different culture substrates, and according to the research results, points out that that the culture medium for axillary bud emergement is same as test-tube seeding propagation, the best medium is MS＋6-BA4 mg/L＋ NAA0.5 mg/L（pH5.8-6.0, sugar3%, agar 0.8%）, the propagation rate of Aloe is 19.6 times a month and 6.4 million times a year,the suitable rooting culture medium is 1/2M S＋ IBA 1.5 mg/L＋ carbon0.3 g/L＋ sugar30 g/L＋ agar7 g/L, and the suitable domestication culture substrate is sand.