| 用比色法测定固定化葡萄糖氧化酶活性的经验公式 |
| |
| 引用本文： | 华家栋,张宪康,杨德荣.用比色法测定固定化葡萄糖氧化酶活性的经验公式[J].应用科学学报,1984,2(3):227-232. |
| |
| 作者姓名： | 华家栋 张宪康 杨德荣 |
| |
| 作者单位： | 上海科学技术大学 |
| |
| 摘 要： | 用包埋法得到的固定化葡萄糖氧化酶,我们发现很难找到一个合适的体系,可用比色法从初速度测定它的活性.提出了一个模拟体系,并得到了该体系的吸收光密度与活性的校正曲线,以解析式A=A0(1-e-βE])表示之.式中A为吸收光密度,E]为葡萄糖氧化酶浓度(活性单位/毫升),A0和β在给定体系下为常数.测得固定化葡萄糖氧化酶在该体系下的A值,便可从该式计算它的活性.
|
| 收稿时间： | 1982-03-05 |
A PRELIMINARY REPORT FOR DETERMINING ACTIVITY OF IMMOBILIZED GLUCOSE OXIDASE BY COLORIMETRY |
| |
| HUA JIADONG,ZHANG XIANKANG,YANG DERONG.A PRELIMINARY REPORT FOR DETERMINING ACTIVITY OF IMMOBILIZED GLUCOSE OXIDASE BY COLORIMETRY[J].Journal of Applied Sciences,1984,2(3):227-232. |
| |
| Authors: | HUA JIADONG ZHANG XIANKANG YANG DERONG |
| |
| Institution: | Shanghai University of Science and Technology |
| |
| Abstract: | It was difficalt to find a suitable system to determine the activity of immobilized glucose oxidase which was produced from entrapping enzyme by means of colorimetry from initail velocity assay. A two-step "Simulate system" and a convenient calibration formula A=A0(1-e-βE]) were given in this paper. Where a was absorbed optical density,E] was the concentration of the immobilized glucose oxidase, A0 and β were constants under a given experimental condition. The more correct activity of immobilized glucose oxidase might be obtained by using the formula mentioned above. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《应用科学学报》浏览原始摘要信息 |
| 点击此处可从《应用科学学报》下载免费的PDF全文 |
|
