左旋精氨酸对大鼠主动脉平滑肌细胞增殖和血管内皮生长因子表达的的影响

观察左旋精氨酸(L-Arg)对大鼠主动脉平滑肌细胞一氧化氮(NO)含量,细胞增殖以及血管内皮生长因子(VEGF)表达的影响,探讨其发生机制。原代培养的大鼠主动脉血管平滑肌细胞分为2组,对照组和L-Arg组。各组培养24 h后检测NO、XTT、VEGFmRNA。L-Arg组NO释放量,VEGF表达量远远高于对照组(P<0.01),但XTT显示细胞增殖活性明显下降(P<0.01)。L-Arg可有效抑制血管平滑肌细胞的增殖、增加NO、VEGF的表达,其机制可能与NO的作用,以及NO与VEGF之间的相互调控有关。

Effects of L-Arg on the Proliferation of Rat Arterial Smooth Muscle Cell and Expression of VEGF

To study the effect of L-Arg on the level of NO and expression of VEGF of cultured rat aortic smooth muscle cells(RASMC) in vitro,and to explore the mechanism of this.RASMC were cultured in vitro.Then the cells were divided into two groups.L-Arg was added to the culture fluid of the test group,and with no L-Arg added group as control.The cells and medium were harvested in 24h to determine NO,XTT and VEGF mRNA.The level of NO and VEGF mRNA were significantly increased in L-arginine group compared with control group(P<0.01),but proliferation of RASMC was decreased in L-arg group(P<0.01).L-arg can decrease the proliferation of RASMC and increase the level of NO and VEGF.The mechanism may be related with NO and VEGF.