基于groEL基因序列鉴定丹毒丝菌属菌种

对4株红斑丹毒丝菌的16SrRNA基因和groEL基因进行测序及系统发育分析,探讨groEL基因序列分析法在丹毒丝菌属菌种分类鉴定中的应用.测序结果表明,4株红斑丹毒丝菌groEL基因长度为1 614bp,编码由537个氨基酸残基组成热休克蛋白HPS60,与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株groEL基因的同源性分别为99%和86%,而16SrRNA基因长度为1 351bp,与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株的同源性均为99%.系统发育分析结果表明,groEL基因比经典的16SrRNA基因序列分析分辨率高,可适用于红斑丹毒丝菌和扁桃体丹毒丝菌的分类鉴定.

Classification and Identification of the Erysipelothrix Species Strains Based on the g roEL Gene Sequences

To investigate the effect of 16S rRNA gene and groEL gene used in the classification and identification of species of the genus Erysipelothrix，the 16S rRNA and groEL genes of four E.rhusiopathiae strains were amplified by PCR and sequenced and their phylogenetic threes was constructed.The open reading frame of groEL genes of four tested strains were 1 614 bp in length，and sequence similarities of the groEL gene between the four tested strains and the previously reported E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 were 99% and 86%，respectively.While the 16S rRNA genes of the four tested strains was 1 351 bp in length，and sequence similarity of the 16S rRNA gene between the four tested strains and E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 was 99%.Phylogenetic analysis indicated that the E.rhusiopathiae and E.tonsillarum were differentiated well by groEL gene sequence analysis.This study demonstrated that the groEL gene is an alternative phylogenetic marker for the classification of species of the genus Erysipelothrix.