| 荧光淬灭法测定人发样中的铜 |
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| 引用本文： | 鲁秀国,范垂生,隋长青.荧光淬灭法测定人发样中的铜[J].松辽学刊,1991(2). |
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| 作者姓名： | 鲁秀国 范垂生 隋长青 |
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| 作者单位： | 四平师范学院，四平师范学院，四平师范学院 四平市人事局 |
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| 摘 要： | 本文研究了在酸性介质中,以荧光素(Flzcorescein)为荧光试剂的荧光淬灭法测定微量铜的方法.建立了最佳测试条件,其最佳激发波长和荧光波长分别为439.7nm和510.3nm,反应的适宜酸度范围pH2.00～7.10,铜含量在0.0～0.2μg/ml范围内成直线关系,回收率为96.5％～98.3％,符合方法学的要求,用于人发中铜含量的测定与原子吸收法比较结果基本一致.
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| 关 键 词： | 荧光素 荧光淬灭法 标准曲线 荧光强度 |
MEASURING COPPER CONTENT IN HUMAN HAIR BY FLOURESCENE EXTINGUISH METHOD |
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| Lu Xiuhuo Fan Chuisheng Shui Changqing.MEASURING COPPER CONTENT IN HUMAN HAIR BY FLOURESCENE EXTINGUISH METHOD[J].Songliao Journal (Natural Science Edition),1991(2). |
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| Authors: | Lu Xiuhuo Fan Chuisheng Shui Changqing |
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| Institution: | Siping Tcarchcr's College |
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| Abstract: | The paper discusses the measuring micro-copper by fluorescene extinguish method taking fluorescein as reagent in acid medium. The paper has established the best measuring conditions: the best stimulating wave length is 439. 7nm; fluorcsccne wave length is 510,3nm; the suitable acid range is pH2. 00-7. 10; the copper content relation is a straight line in the scope of 0. 0-0. 2mg/ml. The rate of recovery is 96. 5%-98. 3%. That is in accordance with methodology. The result of measuring the atom absorption method. |
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| Keywords: | Fluorescein Fluorescene extinguish method Standard curve Fluorescene strength |
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