rhIGF-I对大鼠骨质疏松性骨折愈合过程中OPG表达的影响

目的:观察骨质疏松性骨折愈合不同阶段骨保护素(osteoprotegerin,OPG)的表达,探讨胰岛素生长因子-I(recombinant human insulin-like growth factor,rhIGF-1)对该过程OPG表达的影响.方法:6月龄雌性大鼠40只,制备右股骨骨质疏松性骨折模型后,随机分为IGF治疗组及生理盐水对照组,并分别于术后第10、20、30、40 d取骨痂,通过RT-PCR及ELISA检测各组骨痂OPG表达.结果:在大鼠骨质疏松性骨折愈合过程中成骨细胞活性降低,OPG表达呈弱阳性表达,骨折愈合缓慢;rhIGF-1局部注射治疗能增加骨痂中OPG的表达量(P＜0.05),促进骨质疏松性骨折愈合.结论:OPG是rhIGF-促骨质疏松性骨折愈合的重要下调因子.

Effects of rhIGF-I on osteoprotegerin in the healing process of osteoporotic fracture

Aim:To observe expression of osteoprotegerin(OPG) in the healing process of osteoporotic fracture,and investigate the effect of rhIGF-I on this process.Methods: Six months old SD rats were operated on for osteoporotic fracture model,then randomly divided into two groups,rhIGF-I administration group and control group,RT-PCR and ELISA were used to detect OPG of bone callus,then compare experimental data between two groups at 10th,20th,30th and 40th day ofter operation.Results: The expression of OPG in the healing process of osteoporotic fracture was weak.The healing process of the fracture is slow.Compared with that of the control group,the expression level of OPG in rhIGF-I administration group was significant increase(P<0.05).Conclusion:RhIGF-I promotes osteoporotic fracture healing,OPG act as an important down-media cytokine.