| 接触式低温固定中样品内部传热过程的分析 |
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| 引用本文： | 俞国新,卫宇,华泽钊,任禾盛.接触式低温固定中样品内部传热过程的分析[J].上海理工大学学报,1992(2). |
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| 作者姓名： | 俞国新 卫宇 华泽钊 任禾盛 |
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| 作者单位： | 上海机械学院动力工程学院
(俞国新，卫宇，华泽钊)，上海机械学院动力工程学院(任禾盛) |
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| 摘 要： | 建立了用于电子显微镜生物样品制备的接触式低温固定法中的传热过程模型,并求得了一维常物性条件下的精确解。讨论了样品内部冷却速率的一些变化规律及其与样品初始温度和固化温度的关系,为实际的低温固定操作提供了理论指导。
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| 关 键 词： | 低温电镜技术 低温固定 生物样品制备 传热 |
ANALYSIS ON BIO-SPECIMEN HEAT TRANSFER PROCESS IN IMPACT CRYOFIXATION |
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| Yu Guoxin Wei Yu Hua Zezhao Ren Hesheng.ANALYSIS ON BIO-SPECIMEN HEAT TRANSFER PROCESS IN IMPACT CRYOFIXATION[J].Journal of University of Shanghai For Science and Technology,1992(2). |
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| Authors: | Yu Guoxin Wei Yu Hua Zezhao Ren Hesheng |
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| Institution: | Yu Guoxin Wei Yu Hua Zezhao Ren Hesheng |
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| Abstract: | The heat transfer process in impact cryofixation of bio-specimen preparation for election microscopy is analysized theoretically. An exact solution of the local cooling rate is presented for one-dimensional system with the constant thermal properties. The effects of the initial temperature and melting temperature of bio-specimens on the cooling rate are treated of. The results prove admirably useful in the practice of cryofixaion. |
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| Keywords: | Cryo-electron-microscopy Technique Cryofixation Bio-specimen Preparation Heat transfer |
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