Characterization of Anabaena cylindrica Solution System Using Synchronous-Scan Fluorescence Spectroscopy |
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摘 要: |
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关 键 词: | 项圈藻 水藻 同步扫描荧光光谱技术 腐殖酸 波长 生物化学 铁 |
收稿时间: | 6 March 2004 |
Characterization ofAnabaena cylindrica solution system using synchronous-scan fluorescence spectroscopy |
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Authors: | Liu Xian-li Deng nan-sheng Tao Shu |
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Institution: | (1) College of Environmental Sciences, Peking University, 100871 Beijing, China;(2) College of Resources and Environmental Sciences, Wuhan University, 130079 Wuhan, Hubei, China |
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Abstract: | The characterization of the algaeAnabaena cylindrica solution with Fe (III) was investigated using fluorescence emission and synchronous-scan spectroscopy. The ranges of concentrations
of algae and Fe (III) in aqueous solutions were 5.0×108 2.5×108 cell/L and 1060 μmol/L. respectively. The effective characterization method used was synchronous-scan fluorescence spectroscopy
(SFS). The wavelength difference (Δλ) of 90 nm, was maintained between excitation wavelength (λen) and emission wavelength
(λen). The peak was observed at about (λex) (λem) 326 nm for synchronous scan fluorescence spectroscopy. The fluorescence
quenching in system of algae Fe(III)-HA was studied using synchronous-scan spectroscopy for the first time, Fe(III) was clearly
the effective quencher. The relationship betweenI I (quenching efficiency) andc (concentration of Fe (III) added) was, a linear correlation for the algae solution with Fe(III). Also, Aldrich humie acid
(HA) was found to be an effective quencher.
Foundation item: Supported by the National Natural Science Foundation of China (20177017) and the Scientific Research Foundation of Wuhan
Environmental Protection Bureau
Biography: LIU Xian li(1965), male. Ph.D. Associate professor, research direction: environmental chemistry. |
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Keywords: | fluorescence spectroscopy synchronous scan fluorescence quenching Anabaena cylindrica Fe(III) humic acid |
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