| 农杆菌介导的AtPCS1基因转化陇东苜蓿的初步研究 |
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| 引用本文： | 王鸣刚,骆焕涛,李盼盼.农杆菌介导的AtPCS1基因转化陇东苜蓿的初步研究[J].西北师范大学学报,2010(6). |
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| 作者姓名： | 王鸣刚 骆焕涛 李盼盼 |
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| 作者单位： | 兰州理工大学生命科学与工程学院; |
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| 基金项目： | 甘肃省自然科学基金资助项目(3ZS042-B25-011); 兰州理工大学博士基金资助项目(SB08200602) |
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| 摘 要： | 利用植物表达载体pBI121-AtPCS1转化农杆菌LBA4404,经菌落PCR鉴定获得阳性菌,利用叶盘法以重组的农杆菌侵染陇东苜蓿的子叶.采用GUS组织化学染色和对部分转基因再生植株进行PCR鉴定,初步结果表明AtPCS1基因已成功整合到苜蓿基因组.
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| 关 键 词： | 陇东苜蓿 AtPCS1基因 遗传转化 组织培养 转基因植株 |
The research on agrobacterium-mediated transformation of AtPCS1 into Medicago sativa L |
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| WANG Ming-gang,LUO Huan-tao,LI Pan-pan.The research on agrobacterium-mediated transformation of AtPCS1 into Medicago sativa L[J].Journal of Northwest Normal University Natural Science (Bimonthly),2010(6). |
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| Authors: | WANG Ming-gang LUO Huan-tao LI Pan-pan |
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| Institution: | WANG Ming-gang,LUO Huan-tao,LI Pan-pan(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,Gansu,China) |
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| Abstract: | Agrobacterium LBA4404 are transferred with express plasmid pBI121-AtPCS1 and identified the positive strains by bacterial colony PCR,and AtPCS1 gene is transferred into cotyledon of Longdong alfalfa by leaf infection method.The primary results of histological detection of GUS activity and AtPCS1-specific PCR to some regeneration transgenic plants show that the AtPCS1 has been transferred into alfalfa genome. |
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| Keywords: | Longdong alfalfa AtPCS1 gene genetic transformation tissue culture transgenic plant |
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