| 不同长度马铃薯GBSS基因启动子的块茎专一性表达的初报 |
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| 引用本文： | 宋光光.不同长度马铃薯GBSS基因启动子的块茎专一性表达的初报[J].复旦学报(自然科学版),1998,37(4):559-563. |
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| 作者姓名： | 宋光光 |
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| 作者单位： | 复旦大学遗传工程国家重点实验室 |
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| 基金项目： | 国家科委八六三高科技项目，国家自然科学基金 |
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| 摘 要： | 将０．４，０．８，１．６，２．９ｋｂＧＢＳＳ基因的５’侧翼区与ＧＵＳ基因融合，构建了双元表达载体。０．８ｋｇＧＢＳＳ－ＧＵＳ通过基因枪转化法在块茎切片中获得了瞬间表达。
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| 关 键 词： | 马铃薯 GBSS GUS 块茎专一表达 基因表达 启动子 |
A Preliminary Study on the Tuber-specificity of Truncated Promoters of Potato (Solanum Tuberosum L. )GBSS Gene |
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| Song Dongguang, Huang Daqing, Wang Guangqing, Wang Xunming.A Preliminary Study on the Tuber-specificity of Truncated Promoters of Potato (Solanum Tuberosum L. )GBSS Gene[J].Journal of Fudan University(Natural Science),1998,37(4):559-563. |
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| Authors: | Song Dongguang Huang Daqing Wang Guangqing Wang Xunming |
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| Abstract: | Binary vectors were constructed through fusing 0. 4,0. 8, 1. 6 and 2. 9 kb 5'flanking regions of GBSS gene with the GUS gene. Transient GUS expressions were observed on tuber slices bombarded with the 0. 8 kb GBSS-GUS construct. These constructs were then introduced into potato via Agrobacterium-mediated transformation. Integration of the foreign DNA into the genome was tentatively demonstrated using X-Glue staining and the PCR. Expression levels of the GUS gene under the control of 2. 9, 1. 6 and 0. 4 kb GBSS promoters in microtubers were 1 to 15 times higher than those in stems, with the 2. 9 kb being the most tuber specific. GUS activities were much stronger when the 2. 9 and 1. 6 kb GBSS promoters were used. |
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| Keywords: | Solanum tuberousm L granule-bound starch synthase gene promoter betaglucuronidase tuber-specific expression |
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