| 啤酒废酵母超氧化物歧化酶（SOD）的分离纯化及性质研究 |
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| 引用本文： | 曾仕廉 张宏. 啤酒废酵母超氧化物歧化酶（SOD）的分离纯化及性质研究[J]. 安徽大学学报(自然科学版), 1995, 19(2): 88-92 |
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| 作者姓名： | 曾仕廉 张宏 |
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| 作者单位： | 安徽大学生物系，北京肿瘤研究所 |
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| 摘 要： | 啤酒废酵母（１０８－１０９细胞／ｍｌ）通气发酵后，经乙醇－氯仿沉淀，加热，调ＰＨ，（ＮＨ４）２ＳＯ４沉淀等除去杂蛋白质。最后经ＳｅｐｈａｄｅｘＧ－７５层析得到纯化的ＣｕＺｎ－ＳＯＤ，酶比活在１５００单位／ｍｇ蛋白以上，酶的分子量为１４８０００，由四个亚基组成，紫外最大吸收峰位于２５６ｕｍ处。Ａ２５６．４／２８０＝１．２２．
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| 关 键 词： | 超氧化物歧化酶，啤酒废酵母 |
Purification and Partial Properties of Cupper/Zine Superoxide Dimutase from Yeast(Saccharomyces Cerevisiae) |
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| Zeng Shilian,Zhang Hong. Purification and Partial Properties of Cupper/Zine Superoxide Dimutase from Yeast(Saccharomyces Cerevisiae)[J]. Journal of Anhui University(Natural Sciences), 1995, 19(2): 88-92 |
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| Authors: | Zeng Shilian Zhang Hong |
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| Abstract: | After fermentation by air of waste saccharomyces cerevisiae, predpitation of contaminat proteins with chloroform/ethanol, heat denataration, (NH4)2 SO4 salting out and control of PH value, the predpitated proteins were separated and the SOD contuining solution was purified by gel filtration (sephadex G-75). Said process to give a field of SOD with an activity of 1500 nits/mg, the molecular weight of Cu. Zn--SOD from yeast was 148000 consisted of four Subunits, the enzyme exhibited one absorption maximuxn in the Ultraviolet at 256. 4um, A 256. 4/A 280=1. 22. |
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| Keywords: | superokide dimutase (SOD) saccharomyces cerevisiae |
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