| 谷物中脱氧雪腐镰刀菌烯醇含量测定方法的比较 |
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| 引用本文： | 李亚平,玉崧成,于斐,吴拥军,张洪权.谷物中脱氧雪腐镰刀菌烯醇含量测定方法的比较[J].郑州大学学报(自然科学版),2013(2):76-79. |
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| 作者姓名： | 李亚平 玉崧成 于斐 吴拥军 张洪权 |
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| 作者单位： | [1]郑州大学公共卫生学院,河南郑州450001 [2]郑州大学化学与分子工程学院,河南郑州450001 |
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| 基金项目： | 河南省重大公益性科研招标资助项目,编号101100911300. |
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| 摘 要： | 建立了测定谷物中脱氧雪腐镰刀菌烯醇（DON）含量的酶联免疫吸附测定法（ELISA）和高效液相色谱法（HPLC），利用两种方法对谷物样品进行DON含量测定，并对结果进行了比较．结果表明，ELISA法的检测限为0．043μg/mL，玉米、小麦样品的平均回收率分别为96．8％和97．3％；RSD的平均值分别为8．8％和4．4％；HPLC法的检测限为0．1μg／mL，玉米、小麦样品的平均回收率均为92．7％，RSD的平均值分别为6．8％和3．1％．结果显示，ELISA法相对HPLC法快速简便、回收率高．
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 高效液相色谱 酶联免疫吸附测定 谷物 |
Comparison of Detection Methods for Deoxynivalenol in Grains |
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| LI Ya-ping,YU Song-cheng,YU Fei,WU Yong-jun,ZHANG Hong-quan.Comparison of Detection Methods for Deoxynivalenol in Grains[J].Journal of Zhengzhou University (Natural Science),2013(2):76-79. |
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| Authors: | LI Ya-ping YU Song-cheng YU Fei WU Yong-jun ZHANG Hong-quan |
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| Institution: | 1. College of Public Health, Zhengzhou University, Zhengzhou 450001, China;2. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China) |
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| Abstract: | The two methods （ HPLC and ELISA） for the determination of DON in grains were established and compared. The limit of detection （LOD） of ELISA was 0.043μg/mL, the average recovery rates of corn and wheat samples were 96.8% and 97.3% respectively, with average RSD 8.8% and 4.4%. The LOD of HPLC was 0.1μg/mL, the average recovery rates of corn and wheat samples were both 92.7% , with average RSD 6.8% and 3.1% respectively. The results showed that the ELISA method had higher recovery and simpler sample preparation. |
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| Keywords: | deoxynivalenol high performance liquid chromatography enzyme linked immunosorbent assay grain |
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