| SEPTIC技术快速检测放线菌 |
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| 引用本文： | 李秀丽,刘雨.SEPTIC技术快速检测放线菌[J].复旦学报(自然科学版),2009,48(3). |
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| 作者姓名： | 李秀丽 刘雨 |
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| 作者单位： | 李秀丽,LI Xiu-li(同济大学,附属第十人民医院皮肤科,上海200072);刘雨,LIU Yu(同济大学,附属第十人民医院感染科,上海200072)
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| 摘 要： | 为了评价SEPTIC(sensing of phage-triggered ion cascade)技术在放线菌快速检测中的应用价值.应用纳米阱微芯片检测噬菌体感染放线菌时导致细胞内离子释放发生的微范围内的电位变化.将放线菌和噬菌体等体积混合,孵育后取混合液滴于探针上,采集数据,每2 nm采集一个数据文件,共采集10 min,计算机分析采集结果.结果显示当放线菌与放线菌噬菌体ΦHAU3混合反应时,在10 min内显示了非常明确的l/f功率谱特征,而且功率谱强度明显高于阴性反应的功率谱,并出现了明确的电压超出士4σ范围的波形,持续约O.25 s的时间,可认为发生了1次放线菌被噬菌体感染的事件,并且探针明确地捕获了这一事件.表明SEPTIC技术能在较短时间内检测、鉴定出活菌的存在.
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| 关 键 词： | 放线菌 噬菌体 |
Rapid Detection of Actinomycete by SEPTIC |
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| LI Xiu-li,LIU Yu.Rapid Detection of Actinomycete by SEPTIC[J].Journal of Fudan University(Natural Science),2009,48(3). |
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| Authors: | LI Xiu-li LIU Yu |
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| Institution: | 1.Department of Dermatology;Tenth people's Hospital of Tongji University;Shang hai 200072;China;2.Department of Infection;China |
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| Abstract: | To evaluate the appliance of rapid detection of actinomycete in sampies by sensing of phage-triggered ion cascade(SEPTIC).A nano-well device was used to detect the stochastic spatiotemporal electrical field fluctuationse based on the massive transitory ion leakage that occurs at the moment of phage DNA injection into the host cell.The turbidities of Escherichia coli,actinomycete and corresponding bacteriophage are regulated to certain extents.The tested actinomycete are mixed with an equal volume of the pha... |
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| Keywords: | SEPTIC |
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