| 粉尘螨Der f15的基因克隆与其表达载体的构建 |
| |
| 引用本文： | 李钟鸣,邬玉兰,刘志刚. 粉尘螨Der f15的基因克隆与其表达载体的构建[J]. 江西师范大学学报(自然科学版), 2013, 0(2): 159-161 |
| |
| 作者姓名： | 李钟鸣 邬玉兰 刘志刚 |
| |
| 作者单位： | 深圳大学医学院过敏反应与免疫学研究所,广东深圳,518060 |
| |
| 基金项目： | 国家“863”计划(2006AA02A231);国家自然科学基金(81071388);广东省自然科学基金(04554);广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009);深圳市重点实验室组建项目(SW201110010);深圳市科技计划基础研究重点项目(JCYJ20120613100657482);深圳市南山区一般研发课题(KC2012JSYB0003A)资助项目 |
| |
| 摘 要： | 先挑取纯培养的粉尘螨,提取总的RNA,后采用RT-PCR方法进行反转录出cDNA.由cDNA提取出目的基因进行片段扩增,产物连接入T载体(pMD18-T).经扩增后,用EcoR I和Xho I双酶切,将目的基因分别连接到pET28和pET32的表达载体上,得到重组质粒pET28和pET32,即粉尘螨的基因克隆与 表达载体构建成功.
|
| 关 键 词： | 粉尘螨 Der f15 克隆 载体构建 |
Cloning and Vectorconstuction of Der f15 from House Dust Mite Dermatophagoides Farina |
| |
| LI Zhong-ming,WU Yu-lan,LIU Zhi-gang. Cloning and Vectorconstuction of Der f15 from House Dust Mite Dermatophagoides Farina[J]. Journal of Jiangxi Normal University (Natural Sciences Edition), 2013, 0(2): 159-161 |
| |
| Authors: | LI Zhong-ming WU Yu-lan LIU Zhi-gang |
| |
| Affiliation: | (State Key Laboratory of Respiratory Disease for Allergy,Shenzhen University,Shenzhen Guangdong 518060,China) |
| |
| Abstract: | The living mites of south China area which had been identified and cultured were picked,from which extracted the total RNA.RT-PCR technology were used to clone the gene and then connected it to the T vector(pMD18-T).After that,the expression vector(pET28 and pET32)was constructed for the new gene segment and were connect ed together. |
| |
| Keywords: | dermatophagoides farina Der f15 cloning vectorconstruction |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《江西师范大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《江西师范大学学报(自然科学版)》下载全文 |
