| 聚合膜固定葡萄糖氧化酶及酶活性分析 |
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| 引用本文： | 向灿辉,孙志勇. 聚合膜固定葡萄糖氧化酶及酶活性分析[J]. 井冈山大学学报(自然科学版), 2011, 0(3): 65-69 |
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| 作者姓名： | 向灿辉 孙志勇 |
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| 作者单位： | 遵义医学院珠海校区生物工程系;湖南师范大学化学化工学院; |
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| 摘 要： | 采用电化学石英晶振阻抗分析法,测定了中性缓冲溶液中葡萄糖氧化酶在去甲肾上腺素电氧化聚合膜中固定化酶的量;并结合安培法,定量检测了固定化酶(ESAi)和游离酶(ESAn)的比活性以及固定化酶相对于游离酶的酶相对比活性(ERA)。另外,采用扫描电化学显微镜(SECM)监测了酶电极检测电位下"泄漏"H2O2的氧化电流,并分析该泄漏电流的存在可能是引起酶电极ERA偏低的重要原因。
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| 关 键 词： | 电化学石英晶振阻抗分析法 扫描电化学显微镜 聚合膜 酶活性 葡萄糖氧化酶 |
EVALUATION OF THE GLUCOSE OXIDASE'S IMMOBILIZATION AND ITS ENZYMATIC ACTIVITY IN POLYMER MEMBRANE |
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| XIANG Can-hui,SUN Zhi-yong. EVALUATION OF THE GLUCOSE OXIDASE'S IMMOBILIZATION AND ITS ENZYMATIC ACTIVITY IN POLYMER MEMBRANE[J]. Journal of Jinggangshan University(Natural Sciences Edition), 2011, 0(3): 65-69 |
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| Authors: | XIANG Can-hui SUN Zhi-yong |
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| Affiliation: | XIANG Can-hui1,2,SUN Zhi-yong1 (1. School of Biological Engineering,Zhuhai Campus of Zunyi Medical College,Zhuhai,Guangdong,519041,China,2. College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha,Hunan 410081,China) |
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| Abstract: | The ESA; immobilization capacity of glucose oxidase (GOD) grown in neutral buffered solution in the noradrenaline (NA) electronic oxidation polymer membrane was investigated by the electrochemical quartz crystal impedance analysis (EQCIA) method. ERA of ESAi and ESAn,relative ERA of ESAi to ESAn was quantificationally detected by ampere method. The leaking current of H2O2 oxidation at the PFNO-GOD/Au electrode was investigated by a scanning electrochemical microscope (SECM),which is suggested as an importan... |
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| Keywords: | electrochemical quartz crystal impedance analysis (EQCIA) scanning electrochemical microscope (SECM) polymer enzymatic activity glucose oxidase |
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