Muscle fluorometry: A determination of the depth of penetration |
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| Authors: | A W Stewart |
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| Institution: | (1) Department of Physiology, Monash University, 3168 Clayton, Vicroria, (Australia);(2) Present address: Licoln Institute of Health Sciences, 625 Swanston Street, 3053 Carlton, Victoria, Australia |
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| Abstract: | Summary Fluorometric recordings of NADH (nicotinamide adenine dinucleotide) were made on rabbit papillary muscles. The specimens were placed between the UV light source and a small window of the detection stage. As the muscle was moved over the window in a transverse direction, simultaneous measurement could be taken of transmitted UV light and fluorescent light for various thicknesses of tissue. It is concluded that a muscle thinckness of 0.65 mm, is optimal before absorption of the incident light decreases the fluorescence signal. |
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| Keywords: | Rabbit papillary musle muscle fluorometry penetration depth |
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