大黄酚和牛血清蛋白相互作用的电化学性质研究

在pH 4.0的Britton-Robinson(B-R)缓冲体系中,应用循环伏安法和示差脉冲伏安法对大黄酚与牛血清白蛋白(BSA)相互作用的电化学性质进行研究.结果表明,二者结合生成了一种非电活性的超分子化合物,同时对结合反应的机理进行了探讨.BSA的存在导致大黄酚氧化还原峰电流降低,峰电位基本不变,峰电流的下降值同所加入的BSA浓度在一定范围内呈线性关系.线性范围5.0×10-6mol/L～1.0×10-7mol/L,检出限3×10-7mol/L.

Electrochemical Study of the Interaction between Emodin and BSA

In pH 4.0 Britton-Robinson(B-R)buffer solution,electrochemical of the interaction between emodin and bovine serum albumin(BSA) was studied by using cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The results showed that emodin and bovine serum protein generated a electrochemical inactive super-molecular compound.At the same time,the combined reaction mechanism was discussed.The redox peak current of emodin decreased and the electric potential changed little as a result of the presence of BSA....