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| 反相高效液相色谱法分析盘基网柄菌对氨基酸的利用 | |
| 引用本文： | 黄霄红,王颖,叶美玲,罗臻,宋娟,凌雪萍.反相高效液相色谱法分析盘基网柄菌对氨基酸的利用[J].厦门大学学报(自然科学版),2012,51(4):734-739. |
| 作者姓名： | 黄霄红 王颖 叶美玲 罗臻 宋娟 凌雪萍 |
| 作者单位： | 1. 厦门大学医学院,福建厦门,361005 2. 厦门大学化学化工学院,福建厦门,361005 3. 吉林华康药业股份有限公司,吉林敦化,133700 |
| 摘 要： | 为观察盘基网柄菌(Dictyostelium discoideum)在SIH培养基上对氧基酸的利用情况,以2.4-二硝基氯苯为衍生化试剂,研究了用反相高效液相色谱(RP-HPLC)测定氨基酸的方法.在60 min内16种氨基酸达到基线分离,峰面积与氨基酸浓度的线性相关系数为0.992～0.999.对发酵液样品的分析结果表明,盘基网柄菌对赖氨酸的利用最为彻底,发酵后期赖氨酸已被消耗完全.蛋氯酸、色氨酸,精氨酸、组氧酸也较易被盘基网柄菌利用,而对天冬氨酸、谷氨酸、甘氨酸、苏氨酸、脯氨酸、缬氨酸的需求不大.这一代谢特点为改进盘基网柄菌培养基组成提供依据. |
| 关 键 词： | 盘基网柄菌 反相高效液相色谱法 氨基酸 2 4-二硝基氯苯 |
Analysis of Amino Acid Consumption During the Cultivation of Dictyostelium discoideum by Reversed-phase High Performance Liquid Chromatography |
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| HUANG Xiao-hong , WANG Ying , YE Mei-ling , LUO Zhen , SONG Juan , LING Xue-ping.Analysis of Amino Acid Consumption During the Cultivation of Dictyostelium discoideum by Reversed-phase High Performance Liquid Chromatography[J].Journal of Xiamen University(Natural Science),2012,51(4):734-739. | |
| Authors: | HUANG Xiao-hong WANG Ying YE Mei-ling LUO Zhen SONG Juan LING Xue-ping |
| Institution: | 1.Medical College of Xiamen University,2.College of Chemistry and Chemical Engineering,Xiamen University, Xiamen 361005,China;3.Jilin Huakang Pharmaceutical Co.,Ltd.,Dunhua 133700,China) |
| Abstract: | In order to observe amino acid consumption during cultivation of Dictyostelium discoideum on synthetic SIH medium,a method for determination of amino acids by reversed-phase high performance liquid chromatography(RP-HPLC) with 2,4-dinitrochlorobenzene derivatization was investigated.16 amino acids could be well separated in 60 min,and a good linear relationship between peak area and concentration of amino acids was established with the correlation coefficients in the range of 0.992-0.999.Analysis results of samples of D.discoideum on SIH medium indicated that lysine was completely consumed after 7 d of cultivation.Methionine,tryptophan,arginine and histidine were also utilized considerably while aspartic acid,glutamic acid,glycine,threonine,proline and valine did not show significant demand during the cell growth.This metabolic characteristic would supply reliable data to design a more reasonable synthetic medium for D.discoideum. |
| Keywords: | Dictyostelium discoideum reversed-phase high performance liquid chromatography amino acid 2 4-dinitrochlorobenzene |
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