全麻剂作用下Ca~(2 )诱导膜融合的研究

全麻剂通过干扰膜的结构与功能而起作用，研究了四种全麻剂（氯仿、乙醚、乙醇及辛醇）对３ｍｍｏｌＣａ２＋诱导融合影响用ＰＳ：ＤＭＰＣ：Ｃｈｏｌｅｓｔｅｒｏｌ＝５：３：２的人工膜脂质体进行实验，脂质体融合用荧光共振能量转移监测，初步实验表明：在临床浓度范围内，氯仿，乙酸及辛醇能轻微地抑制膜融合，而乙醇在临床浓度附近能促进膜融合，并且随着乙醇浓度的提高其促融合效应变得十分明显，这可能是乙醇分子的极性及通过氢键与磷脂头缔结，并取代部份结构水，减弱水化排斥力而促使膜融合。

Study of Calcium Induced Membrane Fusion with the Action of General Anestheties

The general anesthetics play the action by disordering the construction and fusion of the membrane. The paper studies the effect of four general anesthetics (chloroform, ether, alcohol, octanol) on 3 mmol/L calcium-induced fusion of lipid vesicles composed of PS: DMPC: chloroform = 5:3:2 with suitable fluorescein-labeled phosphatide, the fusion of lipid vesicles was monitored by using fluorescence resonance energy transfer assays. The initial experiment showed, chloroform, ether and octanol can slightly inhibit the membrane fusion within clinical concentration. In contrast to above three anesthetics, alcohol can induce the membrane fusion near clinical concentration. The effect of the induced fusion becomes very apparent, as the concentration increases. This occurs possibly due to the fact that the alcohol molecules have strong polarity, so they may bind to the polar head of phosphatide through hydrogen bond, partly substitute the structure water molecule and deduce the rejection of hydration, so make it easier to get membrane fusion.