| 花生四烯酸产生菌的原生质体诱变育种 |
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| 引用本文： | 周蓬蓬,余龙江,朱敏.花生四烯酸产生菌的原生质体诱变育种[J].华中科技大学学报(自然科学版),2000,28(7):105-106. |
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| 作者姓名： | 周蓬蓬 余龙江 朱敏 |
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| 作者单位： | 周蓬蓬（华中理工大学生命科学与技术学院）;余龙江（华中理工大学生命科学与技术学院）;朱敏（华中理工大学生命科学与技术学院）;梅兴国（华中理工大学生命科学与技术学院） |
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| 摘 要： | 采用化学诱变剂硫酸二乙酯(DES)对由高山被孢霉出发菌株M3-18制备的原生质体进行诱变育种.实验结果表明,采用体积分数为10%的DES诱变3min,可获得高产突变株M20,其花生四烯酸产量比对照株M3-18提高了4.4倍,而且突变株的继代遗传稳定.说明采用原生质体诱变育种是获得花生四烯酸高产菌株的有效方法.
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| 关 键 词： | 高山被孢霉 原生质体 诱变育种 花生四烯酸 |
| 文章编号： | 1000-8616(2000)07-0105-02 |
| 修稿时间： | 2000年1月4日 |
Strain Improvement of Arachidonic Acid-Producing by Protoplast Mutagenagsis |
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| Zhou Pengpeng,Yu Longjiang,Zhu Min,Mei Xingguo.Strain Improvement of Arachidonic Acid-Producing by Protoplast Mutagenagsis[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2000,28(7):105-106. |
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| Authors: | Zhou Pengpeng Yu Longjiang Zhu Min Mei Xingguo |
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| Institution: | Zhou Pengpeng Yu Longjiang Zhu Min Mei Xingguo |
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| Abstract: | The protoplast DES mutagenesis for more increasing arachidonic acid was studied on Mortierella alpina strain. The experiments showed that the high mutants could by obtained by DES to the protoplasts with 3 minutes. The arachidonic acid content of the mutant M20 was increased by 4.4 times than that of the contrast strain M3 18. The results indicated that the protoplast mutagenesis was a better approach to increase arachidouic acid yield of M. Alpina. |
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| Keywords: | Mortierella Alpina protoplast mutagenesis arachidonic acid |
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