维C银翘片中连翘苷的质量控制方法研究

目的：建立维C银翘片中连翘苷的鉴别和含量测定方法。方法：采用薄层色谱法定性鉴别，展开剂为苯-丙酮-醋酸乙酯-甲醇-水（20：25：30：3：3），碘缸显色。采用高效液相色谱法进行含量测定，色谱条件为Agilent ZORBAX SB C18色谱柱（4．6mm×150mm，5μm），流动相为乙睛-水（23：77），检测波长228nm，流速1．0ml／min，柱温30℃。结果：薄层色谱鉴别斑点清晰，易于识别，连翘苷在0．2～2μg范围内线性关系良好，r=0．9993，平均回收率为98．50％，RSD为1．70％。结论：本法操作简便、快速、分离效果好、稳定性高、重现性好，为全面控制维C银翘片的质量提供了一个可靠的方法。

Determination of Phillyrin in Vitamin C YinQiao Tablets

Objective： To establish a method for the identification and determination of Phillyrin in Vitamin C Yinqiao tablets. Methods： TLC was used for qualitative distinction and the developing solvent was benzene-acetone-ethyl acetatemethanol-water （20：25：30：3：3）, colored by iodine-cylinder. HPLC was used for quantitative determination. The chromatographic conditions were as follows： Agilent ZORBAX SB C18 （4.6×150mm, 5μm）, the mobile phase consisted of aeetonitrile-water （23：77）, the UV wavelength of Phillyrin was 228 nm, the flow rate was 1.0ml/min, and the temperature of column was 30℃. Results： TLC was clear and easy to identify. Phillyrin had good linearity over the range of 0.2μg～ 2μg （r =0.9993）. The average recoveries were 98.50%, and RSD was1.70% . Conclusion： The method was simple, sensitive, accurate, and reproducible and could be used to control the quality of Phillyrin in Vitamin C YinQiao Tablets.