Ecdysteroids inLimulus larvae |
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| Authors: | T C Jegla J D Costlow |
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| Institution: | (1) Department of Biology, Kenyon College, 43022 Gambier, Ohio, USA;(2) Marine Laboratory, Duke University, 28516 Beaufort, North Carolina, USA |
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| Abstract: | Summary Free ecdysteroids were extracted from intermolt and premolt larvae ofLimulus polyphemus in the first posthatch stage, purified by TLC and HRLC, and assayed by RIA and theLimulus bioassay 20-hydroxyecdysone appears to be a principal ecdysteroid and occurs at least 3 times higher a concentration in premolt vs intermolt animals.Supported by NSF grant GB-40620.Acknowledgments. We are indebted to Prof. G. R. Wyatt for the antibody, Prof. K. Nakanishi for use of his laboratory for HRLC separations, and to Dr David King for the3H-ecdysone and for performing radio-TLC analyses. |
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