| 五种蓝舌病毒的分子系统 |
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| 引用本文： | 项明.五种蓝舌病毒的分子系统[J].贵州科学,2003,21(1):14-16. |
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| 作者姓名： | 项明 |
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| 作者单位： | 贵州科学院,贵州,贵阳,550001 |
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| 摘 要： | 对蓝舌病毒(BTV)血清型2、11和13的双链RNA中L3基因的全序列进行了测定，该基因编码这种病毒的重要内壳蛋白质(VP3)，应用前人测定的血清型10和17的BTV的L3基因序列，共5种美国存在的BTV的L3基因。该病毒的每种L3基因片断都有2772个核苷酸，包含一个开放读框(ORE)。这个开放读框(ORF)编码由901个氨基酸组成的蛋白质，VP3(分子量103kD)，其等电点为6.0。用这5种蓝舌病毒血清型的核苷酸和氨基酸序列进行分子系统学分析发现，BTV-2血清型与BTV-10、11、13和17的距离较远，美国的五种血清型BTV可分为两个不同的类群。
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| 关 键 词： | 蓝舌病毒 分子系统学 L3基因 基因编码 内壳蛋白质 核苷酸 氨基酸 |
MOLECULAR PHYLOGENCY OF FIVE BLUETONGUE VIRUSES |
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| Abstract.MOLECULAR PHYLOGENCY OF FIVE BLUETONGUE VIRUSES[J].Guizhou Science,2003,21(1):14-16. |
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| Authors: | Abstract |
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| Abstract: | The complete nucleotide sequences of cognate L3 double-stranded RNA (ds-RNA) segments of bluetongue virus (BTV) serotypes 2, 11and 13 encoding the major viral inner capsid protein, VP3 were determined. Each cognate L3 segment was 2772 nucleotides long and contained an open reading frame (ORF). The ORF encode the 901 amino acid VP3 protein ( 103 kD) with a calculated isoelectric point of 6. The molecular phylogenetic analysis using the nucleotide and amino acid sequences of L3 cognate gene of the five BTV serotypes indicated that the BTV-2 was more distantly related than BTV-10, 11, 13 and 17. The five US BTV serotypes were apparently derived from two distinct gene pools. |
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| Keywords: | bluetongue virus molecular phylogency BTV L3 cognate gene segment |
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