| Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture |
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| 作者姓名： | 宁红 Gao Rong QIN Zhen Wang Lihuan LIU Shigui |
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| 作者单位： | [1]NationalLab.ofBiocontrolEngineeringofGrasslandPestsofSichuanUniversity,Chengdu610064,P.R.China [2]PlantQuarantinestationofSichuanAgriculturalDepartment,No.4WuhouciStreet,Chengdu610041 |
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| 摘 要： | Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis (SDS-PAGE) patterns of their soluble proteins,Differences exist significantly among different species and the same species.There is a special protein band for Curvularia at relative mobility value 0.177(Rf 0.177) and a special protein band for C.lunata at Rf 0.225,Two kinds of rpimary antibodies to C.lunata are used to indirect ELISA and Avidin-Biotion-peroxidase Complex(ABC) immunoassay to evaluate their detecting sensitivity and specificity,The sensitivity of ABC immuno- assay is higher than indirect ELISA.APAbs to C.lunata is special to C.lunata.
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| 关 键 词： | 玉米 蛋白质 电泳图形 病原体培养 |
Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture |
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| Gao Rong,QIN Zhen,Wang Lihuan,LIU Shigui.Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture[J].High Technology Letters,2002,8(3):11-15. |
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| Authors: | Gao Rong QIN Zhen Wang Lihuan LIU Shigui |
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| Abstract: | Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophores is(SDS-PAGE)patterns of their soluble proteins. Differences exist significantly among different species and the same species.There is a special protein band for Curvularia at relative mobility value 0.177 (Rf 0.177)and a special protein band for C.lunata at Rf 0.225.Two kinds of primary antibodies to C.lunata are used to indirect ELISA and Avidin Biotinperoxidase Complex (ABC) immun oassay to evaluate their detecting sensitivity and specificity.The sensitivity of ABC immuno-assay is higher than indirectELISA.APAbs to C.lunata is special to C.lunata. |
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| Keywords: | maize Curvularia lunata SDS-PAGE immunological detection |
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