荧光标记磺酸基强聚电解质的合成

为了考察聚电解质溶剂化层的变化，合成了发色团分别为５－（Ｎ，Ｎ－二甲胺基）－１－萘磺酰基（ｄａｎｓｙｌ）和芘（ｐｙｒｅｎｙｌ）基的两种单体。将它们分别与２－丙烯酰胺基－２－甲基丙磺酸（ＡＭＰＳ）、Ｎ，Ｎ－二基丙烯酰胺（ＤＭＡＡ）共聚，得到了ｄａｎｓｙｌ单体摩尔分数为０．００２或ｐｙｒｅｎｙｌ单体摩尔分数为０．００１、ＡＭＰＳ摩尔分数为０．０３－０．９０的两个系列的荧光标记强聚电解质。水溶液的荧光光

Syntheses of Pyrenyl and Dansyl Labeled Strong Polyelectrolytes with Sulfonate Groups

Dansyl monomer N_2_5_(N,N_dimethylamino)_1_naphthalenyl]sulfonyl]amino]ethyl]_2_propenamide (DANSAEP) and pyrenyl monomer N_(1_pyrenylmethyl)methacrylamide (PyMA) were prepared as fluorescent labels. Labeled strong polyelectrolytes were synthesized by copolymerization of 2_acrylamido_2_methylpropane sulfonic acid (AMPS) and dimethyl acrylamide (DMAA) with DANSAEP of mole fraction of 0.002 in water or with PyMA of mole fraction of 0.001 in H 2O/DMSO (3/2 in volume), respectively. Mole fraction of AMPS monomer in copolymer varied from 0.03 to 0.90. The fluorescence spectra in aqueous solution showed a blue_shift of 9 nm for dansylated polyelectrolytes compared with DANSAEP and a decrease in the relative intensity I 1/I 3 of the first to third emission bands for pyrenyl polyelectrolytes to 1.54 from 1.88 for PyMA. These results indicate that the hydrophobicity of macromolecular backbone reduce the polarity surrounding the labeled chromophores even with strong polyelectrolytes.