| PCR—RFLP analysis of mitochondrial DNA ND5／6 region among3 subspecies of common carp（Cyprinus carpio L.）and its application to genetic discrimination of subspecies |
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| 作者姓名： | ZHOUJianfeng WANGZhongwei YEYuzhen WUQingjiang |
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| 作者单位： | StateKeyLaboratoryofFreshwaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China |
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| 基金项目： | the Na-tional Natural Science Foundation of China (Grant No. 39830300) and the Key Laboratory of Freshwater Ecology and Biotechnology，Institute of Hydrobiology，the Chinese Academy of Sciences |
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| 摘 要： | Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio,Cyprinus carpio haematopterus and Cyprinus carpio rubrofuscus) of common carp(Cyprinus carpio L.)A total of 2.4kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently.The results indicated that each subspecies owned one hyplotype and four restriction enzymes(Dde Ⅰ，HaeⅢ,Taq Ⅰ and MboⅠ)produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp.
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| 关 键 词： | 鲤鱼 亚种 遗传鉴别 线粒体DNA 聚合酶链反应 RFLP分析 分子标记 |
| 收稿时间： | 2002-11-06 |
PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies |
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| ZHOUJianfeng WANGZhongwei YEYuzhen WUQingjiang.PCR-RFLP analysis of mitochondrial DNA ND5/6 region among 3 subspecies of common carp (Cyprinus carpio L.) and its application to genetic discrimination of subspecies[J].Chinese Science Bulletin,2003,48(5):465-468. |
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| Authors: | Zhou Jianfeng Wang Zhongwei Ye Yuzhen Wu Qingjiang |
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| Institution: | e-mail: qjwu@ihb.ac.cn |
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| Abstract: | Mitochondrial DNA ND5/6 region was studied by PCR-RFLP analysis among ten representative strains belonging to three subspecies (Cyprinus carpio carpio, Cyprinus carpio haematopterus and Cyprinus carpio rubrofuscus) of common carp (Cyprinus carpio L.). A total of 2.4 kb fragment was amplified and subjected to restriction endonuclease analysis with nine restriction endonucleases subsequently. The results indicated that each subspecies owned one hyplotype and four restriction enzymes (DdeⅠ, HaeⅢ, TaqⅠand MboⅠ) produced diagnostic restriction sites which could be used for discriminating the three subspecies and as molecular genetic markers for assistant selective breeding of common carp. |
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| Keywords: | Cyprinus carpio subspecies ND5/6 PCR-RFLP mo-lecular marker |
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