响应面法优化槲蕨总黄酮提取工艺及其抗氧化活性

为了优化槲蕨总黄酮的提取工艺并研究其抗氧化活性,该文以料液比、乙醇体积分数、超声处理功率和超声辅助提取时间为考察因素,以槲蕨总黄酮产率为研究目标,在单因素实验的基础上运用响应曲面分析法优化槲蕨总黄酮的提取工艺,并探讨了超声波提取的槲蕨总黄酮的抗氧化活性.研究结果表明:超声波辅助乙醇提取槲蕨总黄酮的最佳工艺是料液比为1:30.00(g·mL^-1)、超声功率为450.00 W、乙醇体积分数为68.00%,在该优化条件下槲蕨总黄酮的产率为1.452 mg·g^-1.抗氧化研究表明:槲蕨总黄酮对羟自由基、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和超氧阴离子(O^-₂·)具有较强的清除能力,这说明槲蕨总黄酮具有良好的抗氧化活性.

The Optimization of Extraction Technology of Total Flavonoids from Dryopteris roosii by Response Surface Methodology and Its Antioxidant Activity

For optimizing the extraction process of total flavonoids from Drynaria roosii and determined its antioxidant activity,taking extraction yield of total flavonoids as the objective and solid-liquid ratio,ethanol volume fraction,ultrasonic power and ultrasonic-assisted extraction time as main factors,response surface methodology are conducted to acquire the optimum extraction process of total flavonoids from Drynaria roosii on the basis of single factor test.At the same time,the antioxidant activity of total flavonoids extrated by ultrasonic is evaluated.The result shows that the optimum conditions of ultrasonic-assisted ethanol extraction are as follows.Solid-liquid ratio is 1:30.00(g·mL^-1),ultrasonic power is 450.00 W,and ethanol volume fraction is 68.00%.Under these conditions,the extraction yield of total flavonoids is 1.452(mg·g^-1),which is consistent with the predict values.The antioxidant study shows that the total flavonoids from Drynaria roosii has obviously hydroxyl radicals,1,1-diphenyl-2-picrylhydrazyl(DPPH·)and superoxide anion(O^-₂·)scavenging ability,which suggests it has good antioxidant activity.