| 黑种草籽调节黑素合成的药效物质基础 |
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| 引用本文： | 范雪琦,徐建国,廖莎,周佳,杨伟峻,尚靖.黑种草籽调节黑素合成的药效物质基础[J].吉林大学学报(理学版),2011,49(4):787-791. |
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| 作者姓名： | 范雪琦 徐建国 廖莎 周佳 杨伟峻 尚靖 |
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| 作者单位： | 1. 中国药科大学 新药筛选中心, 南京 210009； 2. 新疆维吾尔自治区卫生厅, 乌鲁木齐 830001；3. 新疆维吾尔自治区药物研究所, 乌鲁木齐 830004 |
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| 基金项目： | 国家科技支撑项目，教育部“新世纪优秀人才支持计划”项目，国家“十一五”科技重大专项基金 |
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| 摘 要： | 以高效液相色谱( HPLC)法对黑种草籽不同体积分数的醇提取物进行色谱峰表征,以体外培养的小鼠B16黑素瘤细胞为模型,采用MTT法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞增殖的影响,采用L-DOPA氧化法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞酪氨酸活性的影响,采用NaOH裂解法测定黑种草籽不同...
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| 关 键 词： | 黑种草籽 细胞增殖 酪氨酸酶活 黑素合成 相关分析 |
| 收稿时间： | 2010-12-30 |
Pharmacodyamic Material Basis of Nigella sativa L.Regulating Melanogenesis |
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| FAN Xue-qi,XU Jian-guo,LIAO Sha,ZHOU Jia,YANG Wei-jun,SHANG Jing.Pharmacodyamic Material Basis of Nigella sativa L.Regulating Melanogenesis[J].Journal of Jilin University: Sci Ed,2011,49(4):787-791. |
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| Authors: | FAN Xue-qi XU Jian-guo LIAO Sha ZHOU Jia YANG Wei-jun SHANG Jing |
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| Institution: | 1. New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China;2. Bureau of Health, Xinjiang Uyghur Autonomous Region, Urumqi 830001, China;3. Xingjiang Institute of Material Medical, Urumqi 830004, China |
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| Abstract: | Chromatographic peaks of different concentration of Nigella sativa L.extacts were characterized by HPLC. B16 murine melanoma cellswere used as an in vitro cultured model. MTT was used to study the effect of Nigella sativa L.ethanolic extracts on the proliferation of B16 murine me
lanoma cells. Oxidation rate of L DOPA was measured to estimate the effect of Nigella sativa L.ethanolic extracts on tyrosinase activity of B16 murine melanoma cells. NaOH cleavage method was performed to study the effect of Nigellasativa L.ethanolic extracts on the melanogenesis of B16 murine melanoma cells. Statistical analysis to the chromatographic peaks of different concentration of Nigella sativa L.extacts and pharmacodynamic data for correlation analysis. Results show that 50% ethanolic extract of Nigella sativa L.enhances me lanogenesis and growing rate of B16 murine melanoma cells. Component effect relationship shows that there is no significant difference in the 6 chromatographic peaks on pharmacodynamic effect. Pharmacodynamic effect is due to the multiple comonents of Nigella sativa L. |
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| Keywords: | Nigella sativa L proliferation tyrosinase activity melanogenesis correlation analysis |
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