| 单糖薄层层析实验的改进 |
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| 引用本文： | 张瑞英,胡岚岚,武金霞.单糖薄层层析实验的改进[J].实验室科学,2012,15(5):45-46,49. |
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| 作者姓名： | 张瑞英 胡岚岚 武金霞 |
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| 作者单位： | 河北大学生命科学学院,河北保定,071002 |
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| 基金项目： | 河北大学第六批教改项目（项目编号JX06-15） |
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| 摘 要： | 单糖薄层层析是一个重要的生物化学实验,常规喷雾法显色得不到清晰的图谱。比较了几种单糖薄层层析显色方法,结果表明,将显色剂加入展层剂中,展层后烘干,能得到清晰的色谱斑点,且不同的单糖显示不同的颜色,无杂质干扰,重现性好。
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| 关 键 词： | 单糖 薄层层析 显色 |
Improvement of the thin layer chromatography of monosaccharide |
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| ZHANG Rui-ying
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HU Lan-lan
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WU Jin-Xia.Improvement of the thin layer chromatography of monosaccharide[J].Laboratory Science,2012,15(5):45-46,49. |
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| Authors: | ZHANG Rui-ying HU Lan-lan WU Jin-Xia |
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| Institution: | (College of Life Sciences, Hebei University, Baoding 071002, China) |
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| Abstract: | The thin layer chromatography of monosaccharide was very important in biochemistry exper- iments, however, clear spot sometimes could not be observed using general spraying method. In this study, several monosaecharide TLC chromogenic methods were compared, the results showed that clear chromatographic spots could be got through adding color reagent into development reagent and drying after development. It had a good reproducible and not be interfered by the impurities, and different monosaceharides showed different color in this method. |
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| Keywords: | monosaccharide thin layer chromatography color |
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