| 转染野生型p53基因对人白血病细胞系生长的影响 |
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| 引用本文： | 刘经纬.转染野生型p53基因对人白血病细胞系生长的影响[J].东莞理工学院学报,2008,15(3):91-93. |
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| 作者姓名： | 刘经纬 |
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| 作者单位： | 华东理工大学,生物工程系,上海,200237 |
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| 摘 要： | 目的研究转染野生型p53基因对人白血病细胞的生长影响作用．方法将克隆有野生型p53基因的pUHD10-3质粒，通过Lipofectin转染人白血病细胞株U937、K562、HL60，用四甲基偶氮唑（Mlvr）法观察细胞的生长情况．结果转染野生型p53基因，可使人白血病细胞U937、K562、HL60的生长明显受到抑制，第4天生长抑制率分别是28．6％、44．9％及49．0％．结论转染野生型p53基因能有效抑制人白血病细胞U937、K562、HL60的生长．
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| 关 键 词： | 克隆 有野生型p53基因 抑制 人白血病细胞 |
The Research on the Effect of the Genes Transfected With Wild-type p53 on the Growth of Human Leukemia Cell Lines |
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| LIU Jing-wei.The Research on the Effect of the Genes Transfected With Wild-type p53 on the Growth of Human Leukemia Cell Lines[J].Journal of Dongguan Institute of Technology,2008,15(3):91-93. |
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| Authors: | LIU Jing-wei |
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| Institution: | LIU Jing-wei East Chins University of Science , Technologe,Bioengineerinn Departmant,Shanghai 200237,China |
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| Abstract: | Research goal:To do research on the effect of the genes transfected with wild-type p53 on the growth of human Leukemia cell lines.Research method:Transfect human Leukemia cell lines U937,K562 and HL60 with the plasmid cloned with wild-type p53 through Lipofectin and then observe their growth using 4-methy 1 law.Research result: It is apparently observed that the growth of human Leukemia Cell Lines U937,K562 and HL60 are inhibited by the genes transfected with wild-type p53 with the growth-inhibited rates va... |
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| Keywords: | Genes transfected with wild-type P53 control human Leukemia cell lines |
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