一株高效絮凝剂产生菌的分离和鉴定

应用多相分类手段对絮凝菌株XHUA9进行了分类研究,并对分离提纯的发酵产物进行紫外和红外分析.基于16S rRNA基因序列的系统发育分析表明,该菌和模式菌株Paenibacillus hunanensis的序列同源性为96.7%,基因组DNA-DNA同源性杂交值为51.6%.菌株的主要极性脂肪酸为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇.菌株优势醌为MK-7,主要优势脂肪酸为anteiso-C15:0和C16:0.菌株(G+C)摩尔分数为51.9%.根据上述结果,菌株XHUA9鉴定为类芽孢杆菌属内的新种,命名为Paenibacillus shenyangensis sp.nov..紫外扫描和红外检测等结果表明,菌株XHUA9所产絮凝剂的主要成分为多糖类物质.

Isolation and Identification of an Efficient Bioflocculant-Producing Strain

In order to ascertain taxonomic position of strain XHUA9, the polyphasic taxonomical identification was performed. The ferment products were separated and purified and then were analyzed by ultraviolet scanning spectrum (UV scanning spectrum) and infrared spectrum (IR spectrum). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus, sharing a most closely sequence similarity of 96.7% and DNA-DNA hybridization value of 51.6% with the type strain Paenibacillus hunanensis. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinone is MK-7 and the major cellular fatty acids were anteiso-C_15:0 and C_16:0. The genomic DNA G+C content was 51.9 mol%. Based on the results of the present study, it is concluded that strain XHUA9 represents a novel species of the genus Paenibacillus, for which the name Paenibacillus shenyangensis sp. nov. is proposed. The UV scanning and IR spectra data showed that bioflocculant produced by strain XHUA9 was mainly composed of polysaccharides.