2-KGA混菌发酵过程的结构模型及实验验证

探讨了古龙酸混菌发酵过程中普通生酮古龙酸菌(Ketogulonicigenium vulgare)和巨大芽孢杆菌(Bacillus megaterium)的交互作用机制.分析了K. vulgare以L 山梨糖为碳源时的代谢途径，建立了K. vulgare的宏观动力学模型，引入了一阶闭环调节器模型来描述糖酵解等酶系的建立过程.该模型描述了K. vulgare中物质流和能量流的平衡关系，据此可解得比碳源消耗速率、比生长速率、比乙酰辅酶A生成速率、比氧消耗速率等关键反应速率.结合生物反应器模型可获得操纵变量与状态变量之间的关系.实验验证了上述模型的有效性.

Structured Modeling and Validation for 2-KGA Mixed Cultivation

The mechanism of interaction between Ketogulonicigenium vulgare and Bacillus megaterium was investigated for 2-keto-L-gulonic acid (2-KGA) mixed culture. Based on the metabolic pathway analysis in K. vulgare, a macrokinetic model was constructed, where, a first-order closed loop regulator model was introduced to describe the induction of the enzyme pool responsible for the growth of K. vulgare at the beginning of the cultivation. The model describes the balance of intracellular substance and energy flow rates. The key reaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetyl-CoA formation rate as well as the specific oxygen uptake rate are obtained with this model. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the relationship between substrate feeding rate and state variables is set up. The experimental validation results were presented.