| 血管生长素衍生物His13Gly的基因工程研究 |
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| 引用本文： | 盛继群.血管生长素衍生物His13Gly的基因工程研究[J].复旦学报(自然科学版),1998,37(4):413-418. |
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| 作者姓名： | 盛继群 |
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| 作者单位： | 复旦大学(盛继群，裘敏燕，刘建平，江广平，谢毅)，长海医院(应康) |
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| 基金项目： | 利诚－复旦生物高新技术研究发展基金 |
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| 摘 要： | 在人工合成的血管生长素基因的基础上，利用化学合成的寡聚核苷酸片段，采用掺Ｕ法对ＡＮＧ知性定位眯诱变，同位素杂交筛选到ＡＮＧ基因的衍生物ＩＳ１３Ｇｌ６突变子。
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| 关 键 词： | 血管生长素 定点诱变 衍生物 基因工程 |
Genetic Engineering Research of Angiogenin Derivative His13Gly |
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| Sheng Jiqun, Ying Kang, Qiu Minyan, Liu Jianping, Jiang Guangping, Xie Yi.Genetic Engineering Research of Angiogenin Derivative His13Gly[J].Journal of Fudan University(Natural Science),1998,37(4):413-418. |
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| Authors: | Sheng Jiqun Ying Kang Qiu Minyan Liu Jianping Jiang Guangping Xie Yi |
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| Abstract: | On the base of chemically synthesizing angiogenic (ANG) gene, the highly expressing recombinant mutant-TG1/PBV220/ANG (rh') have been obtained by utilizing uracil-containing site-directed mutagenesis with mutagenic oligonucleotides and radiolabeled probe blot. Applied this mutant, the expressed angiogenin is about 36% of the total bacterial protein. After the purificatory steps of isolating inclusion body, utrasonic cleaning inclusion body, ion-exchange chromatography and molecular-sieve chromatography, the angiogenin purity was made up to 95%. Angiogenin was renatured by glutathione reoxidation. Applied embryo chroricallantoic membrane to examine the renature efficiency, the biological activity of competitively restrainging the wild type of angiogenin and the level of this activity. |
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| Keywords: | agiogenin site-directed mutagenesis induced expression purification renaturation embryo chorioallantoic membrane |
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