Variants modulating the expression of a chromosome domain encompassing PLAG1 influence bovine stature |
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Authors: | Karim Latifa Takeda Haruko Lin Li Druet Tom Arias Juan A C Baurain Denis Cambisano Nadine Davis Stephen R Farnir Frédéric Grisart Bernard Harris Bevin L Keehan Mike D Littlejohn Mathew D Spelman Richard J Georges Michel Coppieters Wouter |
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Institution: | Unit of Animal Genomics, Interdisciplinary Institute of Applied Genomics (GIGA-R) and Faculty of Veterinary Medicine, University of Liège (B34), Liège, Belgium. |
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Abstract: | We report mapping of a quantitative trait locus (QTL) with a major effect on bovine stature to a ~780-kb interval using a Hidden Markov Model-based approach that simultaneously exploits linkage and linkage disequilibrium. We re-sequenced the interval in six sires with known QTL genotype and identified 13 clustered candidate quantitative trait nucleotides (QTNs) out of >9,572 discovered variants. We eliminated five candidate QTNs by studying the phenotypic effect of a recombinant haplotype identified in a breed diversity panel. We show that the QTL influences fetal expression of seven of the nine genes mapping to the ~780-kb interval. We further show that two of the eight candidate QTNs, mapping to the PLAG1-CHCHD7 intergenic region, influence bidirectional promoter strength and affect binding of nuclear factors. By performing expression QTL analyses, we identified a splice site variant in CHCHD7 and exploited this naturally occurring null allele to exclude CHCHD7 as single causative gene. |
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