| Repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody response |
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| 作者姓名： | LIU Zuqiang WANG Zuguang CHEN Yinghua |
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| 作者单位： | Laboratory of Immunology of Biology,Tsinghua University;Protein Science Laboratory of MOE, Beijing 100084, China,Laboratory of Immunology of Biology,Tsinghua University;Protein Science Laboratory of MOE, Beijing 100084, China,Laboratory of Immunology of Biology,Tsinghua University;Protein Science Laboratory of MOE, Beijing 100084, China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金 |
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| 摘 要： | Based on the hypothesis suggested by us that epitope-vaccine may be a new strategy against HIV mutation, we have studied several neutralizing epitopes on HIV envelope proteins. However we do not know whether a repeated epitope in a recombinant epitope-peptide can enhance epitope-specific antibody response or not. ELDKWA-epitope (aa669-674) on the C-domain of HIV-1 gp41 is a neutralizing epitope defined by the monoclonal antibody (mAb) 2F5 with broad neutralizing activity. In this study, we designed and prepared a series of the recombinant epitope-peptides bearing 1, 4 and 8 copies of ELDKWA-epitope respectively. In the comparison of the antisera induced by the three recombinant antigens, an obviously increased titre of ELDKWA-epitope-specific antibody was observed in the case of four and eight repeated epitopes. In flow cytometry analysis, the epitope-specific antibodies in both antisera showed stronger activity to bind the transfected CHO-WT cells that stably express HIV-1 envelope glycoprotein on the cell surfaces. These experimental results indicated that repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody response, which could contribute to designing an effective recombinant epitope-vaccine.
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| 关 键 词： | neutralizing epitope epitope vaccine antibody response repeated epitope HIV-1 |
Repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody response |
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| LIU Zuqiang,WANG Zuguang,CHEN Yinghua.Repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody response[J].Progress in Natural Science,2005,15(1):89-92. |
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| Authors: | LIU Zuqiang Wang Zuguang CHEN Yinghua |
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| Institution: | Laboratory of Immunology of Biology,Tsinghua University;Protein Science Laboratory of MOE, Beijing 100084, China |
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| Abstract: | Based on the hypothesis suggested by us that epitope-vaccine may be a new strategy against HIV mutation, we have studied several neutralizing epitopes on HIV envelope proteins. However we do not know whether a repeated epitope in a recombinant epitope-peptide can enhance epitope-specific antibody response or not. ELDKWA-epitope (aa669-674) on the C-domain of HIV-1 gp41 is a neutralizing epitope defined by the monoclonal antibody (mAb) 2F5 with broad neutralizing activity. In this study, we designed and prepared a series of the recombinant epitope-peptides bearing 1, 4 and 8 copies of ELDKWA-epitope respectively. In the comparison of the antisera induced by the three recombinant antigens, an obviously increased titre of ELDKWA-epitope-specific antibody was observed in the case of four and eight repeated epitopes. In flow cytometry analysis, the epitope-specific antibodies in both antisera showed stronger activity to bind the transfected CHO-WT cells that stably express HIV-1 envelope glycoprotein on the cell surfaces. These experimental results indicated that repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody response, which could contribute to designing an effective recombinant epitope-vaccine. |
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| Keywords: | neutralizing epitope epitope vaccine antibody response repeated epitope HIV-1 |
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