通过pR1aS导肽提高植物中绿色荧光蛋白的激发强度

绿色荧光蛋白在植物细胞胞质表达中对转化植物细胞的再生存在不利的影响,为增强绿色荧光蛋白在植物细胞中的表达,在mgfp的5'端连接了pR1aS信号肽序列,同时在3'末端引入滞留在内质网中的特异序列KDEL,成功构建了植物表达载体pBLG-pR1aS-gfp,经转基因烟草表达分析,结果表明:转化体植株显著增加了绿色荧光蛋白在烟草中的表达,同时消除了绿色荧光蛋白对植物潜在的光毒害。这为植物转基因转化频率的研究和转基因植物安全性评价提供了一种有利的工具。

To Increase GFP Provocation Strength Through the Introduction of Peptide pR1aS

In the cytoplasm it play an adverse role GFP regeneration of transformed plant cells.To enhance expression of GFP in plant cells,we successfully constructed the plant expression vector pBLG-pR1aS-gfp,in which mGFP introduces a signal peptide sequence of pR1aS at the 5 end and a special sequence of KDEL at the 3' end.The result confirmed that GFP was significantly increased in transgenic tobacco,and simultaneously eliminated the potential light toxicity to plants.It provides a convenient tool for the study o...