表达雪花莲外源凝集素基因的油菜转基因植株的获得

利用农杆菌素LBA4404（pCAMBIA3300RG)转化优良甘蓝型油菜恢复系W723的下胚轴节段.pCAM-BIA3300RG含有Rssl启动子引导的雪花莲外源凝集素基因（gna）和CaMV-35S启动子引导的除草剂抗性基因（bar）。经过两轮除草剂（2.5mg/L bialaphos）筛选（两周/轮），除草剂抗性再生芽被传入生根培养基中生根，对根系旺盛生长的植株中所含gna基因进行PCR分析，PCR分析证实了这些植株确为转基因植株，利用Western印迹法对随机选择的5株含gna基因的转基因植株的分析发现，其中4株表达了gna基因。目前正对这些表达gna基因的转基因植株进行后代遗传分离分析。

Production of Transgenic Rape ( Brassica napus L.) Plants Expressing Snowdrop Lectin ( Galanthus nivalis Agglutinin) Gene

Hypocotyl segments of an elite rape ( Brassica napus L. ) restorer line, W723, were transformed with the Agrobacterium tumefaciens strain LBA4404 (pCAMBIA3300RG). pCAMBIA3300RG contained the snowdrop lectin gene (gna) driven by the Rice Sucrose Synthase 1 (Rss1) promoter and the herbicide-resistance gene (bar)under the control of the CaMV-35S promoter. After 2 rounds of herbicide (2.5 ng/L bialaphos) selection (2 weeks/round), the regenerated herbicide-resistant shoots were transferred to rooting medium. Plants with vigorously growing roots were subjected to PCR analysis for the presence of the gna gene. PCR analysis confirmed their transgenic status. Western blot analysis revealed that 4 out of 5 randomly selected gna-containing tranegenic plants expressing the GNA. These GNA-expressing transgenic plants are undergoing segregation analysis for the transgenes in the following generations.