| 滇紫草愈伤组织辐照和磁场效应研究初报 |
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| 引用本文： | 王曼丝,李鸣镝,曹日强,杨正举,许自然,鹿牧. 滇紫草愈伤组织辐照和磁场效应研究初报[J]. 南京大学学报(自然科学版), 1994, 0(1) |
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| 作者姓名： | 王曼丝 李鸣镝 曹日强 杨正举 许自然 鹿牧 |
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| 作者单位： | 江苏省植物研究所，南京大学 |
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| 摘 要： | 本文初步研究了Ｈｅ—Ｎｅ激光和磁场对滇紫草愈伤组织生长及色素形成的影响．结果表明：功率密度０．３７ｍｗ／ｃｍ２的Ｈｅ－Ｎｅ激光处理１ｈ刺激愈伤组织生长，而色素含量在２ｈ和３ｈ处理有较大提高，分别达１５．７％和２７．８％．１０ＫＧＳ场强的磁场处理１５ｍｉｎ对愈伤组织生长和色素形成均有促进，当代和第二代色素含量较高９．５％和２７．５％。色素含量提高的稳定正在进一步观察．
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| 关 键 词： | 滇紫草，愈伤组织，紫草色素，磁场，He—Ne激光，生物刺激作用 |
EFFECT OF He-Ne LASER AND MAGNETIC FIELD IN ONOSMA PANICULATUM B. et F. CALLUS CULATURE |
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| Wang Mansi, Li Mingdi, Cao Riqiang, Yang Zhengju, Xu Ziran, Lu Mu. EFFECT OF He-Ne LASER AND MAGNETIC FIELD IN ONOSMA PANICULATUM B. et F. CALLUS CULATURE[J]. Journal of Nanjing University: Nat Sci Ed, 1994, 0(1) |
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| Authors: | Wang Mansi Li Mingdi Cao Riqiang Yang Zhengju Xu Ziran Lu Mu |
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| Abstract: | This paper is a preliminary study of the effects of He-Ne laser and Magnetic field on callus growth and shikonin derivatives formation in Onosma paniculatum Bur. et Franch callus culture. Results show that callus growth was promoted when exposed to He-Ne laser with power 0.37mw/cm2at 1h, while content of shikonin derivatives increased 15.7% 27.8% at 2 & 3hs. The magnetic field stimulated both the callus growth and the biosynthesis of shikonin derivatives by 10KGS, 15' treatment. The. contemporay and subsequent passage content of shikonin derivatives increased by 9.5% & 27.5%. The stable increase of shikonin derivatives content is being observed |
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| Keywords: | Onosma Paniculatum Bur. et Franch. callus Shikonin He-Ne laser Magnetic field effect of biological stimulation |
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