| 豫道地金银花化学指纹图谱研究 |
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| 引用本文： | 李建军,贾国伦,李军芳,范晗,梁建强,刘保彬,杨丽.豫道地金银花化学指纹图谱研究[J].河南师范大学学报(自然科学版),2013(1):130-133. |
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| 作者姓名： | 李建军 贾国伦 李军芳 范晗 梁建强 刘保彬 杨丽 |
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| 作者单位： | 河南师范大学生命科学学院,河南省高校道地中药材保育及利用工程技术研究中心;新乡佐今明制药股份有限公司;封丘县贾庄金银花种植专业合作社;封丘县农村科技研究开发推广中心 |
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| 基金项目： | 河南省重点科技攻关项目(112102310019);河南省教育厅自然科学研究计划项目(2011A180017);新乡市重点科技攻关计划项目(ZG12009) |
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| 摘 要： | 目的:建立河南道地金银花药材HPLC标准指纹图谱.方法:高效液相色谱法,色谱柱填充剂为十八烷基硅烷键合硅胶,检测波长265nm,进样量10μL,流动相A为乙腈和流动相B为1%的醋酸水溶液进行梯度洗脱,流速为0.8mL/min.结果:该方法能够很好地分离河南道地金银花的各类成分;不同产地金银花药材指纹图谱相似度较好,建立了含有15个特征指纹峰的豫道地金银花标准指纹图谱;同时标定了绿原酸和木犀草苷的位置;HPLC标准指纹图谱具有良好的重现性和特征性.结论:本实验建立的金银花药材HPLC指纹图谱可用于对河南道地金银花药材的综合评价和质量控制.
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| 关 键 词： | 金银花 化学指纹图谱 HPLC |
Chemical Fingeprint Chromatogram of Henan Trueborn Flos Lonicerae |
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| LI Jianjun,JIA Guolun,LI Junfang,FAN Han,LIANG Jianqiang,LIU Baobin,YANG Li.Chemical Fingeprint Chromatogram of Henan Trueborn Flos Lonicerae[J].Journal of Henan Normal University(Natural Science),2013(1):130-133. |
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| Authors: | LI Jianjun JIA Guolun LI Junfang FAN Han LIANG Jianqiang LIU Baobin YANG Li |
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| Institution: | 1.College of Life Science,Henan Normal University,Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs,Xinxiang 453007,China;2.Xinxiang Zuo Jinming Pharmaceutical Co.,LTD,Xinxiang 453007,China; 3.Jiazhuang Honeysuckle Planting Professional Cooperatives in Fengqiu country,Fengqiu 453311,China;4.The Research and Development Promotion Center of Rural Science and Technology in Fengqiu country,Fengqiu 453341,China.) |
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| Abstract: | Objective:To build the fingeprint chromatogram of Henan trueborn flos lonicerae.Methods:The HPLC method was used;chromatography conditions were ODS column,CAN and 1% acetic acid as mobile phase,and 265 nm as wavelength.25 ℃ as temprature,and 0.8 ml/min as the flow rate.Results:Samples from different habitats were of high similarity,and fifteen characteristic peaks in the fingeprint chromatogram.It's worthwhile demarcated chlorogenic acid and Luteolin-7-glu.The method accurate with a good reproducibility and characteristic.Conclusion:The fingeprint chromatogram can be uesd in quality evaluation and comprehensive evaluation of Henan trueborn flos lonicerae. |
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| Keywords: | flos lonicerae chemical fingeprint chromatogram HPLC |
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