| 表没食子儿茶素没食子酸酯通过活性氧生成抑制细菌的生长 |
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| 引用本文： | 申羽佳,崔海燕,张东芳,王坤英,李卫国. 表没食子儿茶素没食子酸酯通过活性氧生成抑制细菌的生长[J]. 河南师范大学学报(自然科学版), 2013, 41(1): 115-118 |
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| 作者姓名： | 申羽佳 崔海燕 张东芳 王坤英 李卫国 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 河南省教育厅自然科学基金(2010A180011);河南师范大学生物学一级学科建设基金 |
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| 摘 要： | 表没食子儿茶素-3-没食子酸酯(EGCG)具有显著的抗氧化等生物学作用.运用平板菌落计数法,测定了5、10、20mg/L EGCG抑制细菌生长繁殖的效应,分析了200U/mL过氧化氢酶和80mg/L N-乙酰半胱氨酸对EGCG抑菌作用的影响机制.结果显示EGCG以剂量依赖方式抑制表皮葡萄球菌(S.epidermidis)和大肠杆菌(E.coil)的生长繁殖,而过氧化氢酶和N-乙酰半胱氨酸则减弱EGCG的抑菌作用,表明EGCG抑制细菌生长的作用可能是通过生成活性氧介导的.
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| 关 键 词： | 表没食子儿茶素-3-没食子酸酯 抑菌作用 活性氧 过氧化氢酶 N-乙酰半胱氨酸 |
Antimicrobial Action of Epigallocatechin-3-gallate through Reactive Oxygen Species |
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| SHEN Yujia , CUI Haiyan , ZHANG Dongfang , WANG Kunying , LI Weiguo. Antimicrobial Action of Epigallocatechin-3-gallate through Reactive Oxygen Species[J]. Journal of Henan Normal University(Natural Science), 2013, 41(1): 115-118 |
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| Authors: | SHEN Yujia CUI Haiyan ZHANG Dongfang WANG Kunying LI Weiguo |
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| Affiliation: | (College of Life Science,Henan Normal University,Xinxiang 453007,China) |
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| Abstract: | Epigallocatechin-3-gallate(EGCG) has an important role in anti-oxidation and bactericidal action.In this study,we use the method of plate culture count to examine the antimicrobial activity of EGCG against S.epidermidis and E.coil at 5,10,and 20 mg/L,and to analyze the influence of catalase and N-acetylcysteine(NAC) on the antimicrobial action of EGCG.The results show that EGCG inhibits growth of two bacteria in dose-dependence fashion.Both of catalase and N-acetylcysteine partly eliminate the antimicrobial activity of EGCG.The results suggest that the antimicrobial action of EGCG mediated by reactive oxygen species production. |
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| Keywords: | Epigallocatechin-3-gallate(EGCG) antimicrobial action reactive oxygen species(ROS) catalase n-acetylcysteine(NAC) |
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