| 尖镰孢菌细胞色素P45055A1与NO相互作用的荧光光谱法研究 |
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| 引用本文： | 吴云华,刘 倩. 尖镰孢菌细胞色素P45055A1与NO相互作用的荧光光谱法研究[J]. 中南民族大学学报(自然科学版), 2015, 0(2): 28-32 |
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| 作者姓名： | 吴云华 刘 倩 |
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| 作者单位： | 中南民族大学 生命科学学院，武陵山区特色资源植物种质保护和利用湖北省重点实验室，武汉 430074 |
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| 基金项目： | 湖北省自然科学基金资助项目 (2014CFB381)，中央高校自然科学基金资助项目(CZW14008) |
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| 摘 要： | 以大肠杆菌为宿主表达了尖镰孢菌细胞色素P450 55A1(CYP450 55A1),并采用荧光光谱法研究了其与NO的相互作用.结果表明:在大肠杆菌(DE3)中成功表达了具有酶活性的CYP55A1.在280 nm波长激发下,NO的加入使CYP55A1的荧光逐渐降低,但随温度的变化差异较小;在413 nm波长激发下,NO的加入使CYP55A1的荧光逐渐升高,但加入NADH后其荧光又恢复到原来的值.
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| 关 键 词： | 细胞色素P450 55A1 原核表达 一氧化氮 荧光光谱法 |
The Interaction between Cytochrome P450 55A1 from Fusarium oxysporum and NO by Fluorescence Spectrometry |
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| Wu Yunhua;Liu Qian. The Interaction between Cytochrome P450 55A1 from Fusarium oxysporum and NO by Fluorescence Spectrometry[J]. Journal of South-Central Univ for, 2015, 0(2): 28-32 |
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| Authors: | Wu Yunhua Liu Qian |
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| Affiliation: | Wu Yunhua;Liu Qian;Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China,College of Life Sciences,South Central University for Nationalities; |
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| Abstract: | Cytochrome P450 55A1(CYP55A1) from Fusarium oxysporum was expressed in E.coli, and the interaction between CYP55A1 and NO was studied by fluorescence spectrometry. The results indicated that CYP55A1 were expressed in E.coli (DE3) successfully. Under the excitation at 280 nm, the fluorescence intensity of CYP55A1 decreased gradually with the addition of NO but varied marginally with temperature. Under the excitation at 413 nm, the fluorescence intensity increased gradually with the addition of NO but would recover with the addition of NADH. |
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| Keywords: | cytochrome P450 55A1 prokaryotic expression nitric oxide fluorescent spectrometry |
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