利用空间诱变选育辅酶Q10高产菌

 以空间搭载为诱变手段，筛选可用于工业化生产的辅酶Q10高产菌株。以类球红细菌（Rhodobacter sphaeroides）为出发菌株进行空间搭载，根据菌落形态进行初筛，摇瓶发酵进行复筛，HPLC检测含量等方法筛选辅酶Q10高产菌株。最终得到名为Shenzhou6的突变株产量比对照菌株提高30％，产量可达（0.8+0.02）g/L，经过优化培养可满足工业生产需要。同时也说明空间诱变因其复杂的空间环境条件，可以作为一种诱变手段进行工业化菌样的筛选。

Screening of CoQ10 Highly Productive Mutant Strains by Spaceflight Mutation

Spaceflight mutation was carried out to improve Coenzyme Q10(CoQ10) yield to be utilized in industrial microbial fermentation. CoQ10 high yield mutants were screened from Rhodobacter sphaeroides that was collected from a recoverable satellite after a 15-day flight in space. High yield mutant strains were selected based on colony morphology, including colony size and color, and then screened using the method of shake flask fermentation. The CoQ10 production was measured using HPLC. The CoQ10 production of the mutant strain named"Shenzhou 6" reached (0.8+0.02)g/L, 33％ higher than the control group. The production could be increased with the optimization of fementation conditions and the strain could be utilized in the industrial microbial fermentation. Meanwhile, the result revealed that spaceflight showed multi-factor mutagenic effect on Rhodobacter sphaeroides compared to the traditional single-factor mutagenesis methods. It could increased CoQ10 production of isolated strains, and could be utilized in industrial microbial mutagenesis and breeding in the future.