| 小肽不同高效液相色谱柱前衍生化反应的实验研究 |
| |
| 引用本文： | 谢孟峡,丁雅韵,邵华.小肽不同高效液相色谱柱前衍生化反应的实验研究[J].北京师范大学学报(自然科学版),2002,38(1):100-105. |
| |
| 作者姓名： | 谢孟峡 丁雅韵 邵华 |
| |
| 作者单位： | 北京师范大学分析测试中心,北京,100875 |
| |
| 基金项目： | 意大利国家研究项目;97.02858 CT03; |
| |
| 摘 要： | 分别用氯甲酸-9-芴基甲酯(9-fluorenylmethylchloroformate,FMOC-Cl)、9-芴基甲氧基甲酸琥珀酰亚胺类酯(9-fluorenylmethoxy carbonyl succinimide,FMOC-OSU)和丹磺酰氯(1-dimethylaminonaphthalene-5-sulfonyl-chloride,Dns-Cl)为衍生化试剂对小肽进行了衍生化反应.研究和比较了各种衍生试剂用量、衍生缓冲体系pH条件、衍生化时间等因素对衍生反应的影响及衍生产物的稳定性.结果发现,FMOC-Cl对肽的衍生反应速度快,衍生反应完全,衍生产物稳定.
|
| 关 键 词： | 小肽 氯甲酸-9-芴基甲酯 高效液相色谱 柱前衍生 |
| 修稿时间： | 2001年9月14日 |
EXPERIMENTAL STUDY ON DIFFERENT PRE-COLUMN DERIVATISING REACTIONS OF DIPEPTIDES IN HPLC |
| |
| Xie Mengxia,Ding Yayun,Shao Hua.EXPERIMENTAL STUDY ON DIFFERENT PRE-COLUMN DERIVATISING REACTIONS OF DIPEPTIDES IN HPLC[J].Journal of Beijing Normal University(Natural Science),2002,38(1):100-105. |
| |
| Authors: | Xie Mengxia Ding Yayun Shao Hua |
| |
| Abstract: | The derivatising reactions of peptides are conducted by using 9-fluorenyl-methylchloroformate(FMOC-Cl), 9-fluorenylmethoxy carbonyl succinimide (FMOC-OSU) and 1-dimethylamino-naphthalene-5-sulfonyl chloride (Dns-Cl) as derivatising reagent individually. The influence of the amount of each derivatising reagent used and the pH value of the buffer on the derivatising reactions is investigated. The dynamics of derivatising reactions and the stability of the derivatives are also studied. The results show that the derivatising reaction is fast, complete and has high reproducibility when FMOC-Cl is used as the precolumn derivatising reagent of peptides and the FMOC-peptide derivatives have high stability. |
| |
| Keywords: | dipeptides FMOC-Cl HPLC pre-column derivatising |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
