Inhibition of the sodium pump does not cause a stoichiometric decrease of ATP-production in energy limited fish hepatocytes |
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Authors: | G. Krumschnabel W. Wieser |
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Affiliation: | (1) Institute of Zoology, Department of Ecophysiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, (Austria) |
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Abstract: | In isolated goldfish hepatocytes underaerobic conditions the energy requirement for the sodium pump (calculated from Rb+ flux) is closely matched by the ouabain-sensitive fraction of oxygen consumption, whereas during in vitroanoxia (cyanide inhibition of the electron transport chain) the measured ATP demand of the sodium pump clearly exceeds ouabain-sensitive ATP production by anaerobic glycolysis. We conclude that when the energy status of cells is low, part or all of the ATP spared by the inhibition of a particular function may be used for fuelling other ATP-consuming functions. |
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Keywords: | Goldfish hepatocytes (Na+, K+)-ATPase protein synthesis membrane-metabolic coupling |
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