离体培养条件下12个白榆优良无性系氯化钠盐分抗性筛选的研究

以12个优良白榆无性系为材料,采用培养基中加入不同盐分的方法,对0.3%、0.4%、0.5%、0.6%、0.7%、0.8%氯化钠梯度条件下的白榆无性系离体培养植株生长量进行了测定,排除了外界环境条件变化对白榆无性系耐盐能力的影响,并通过隶属函数法,对12个白榆无性系的耐盐能力进行了分析与评价。结果表明,58号、30号、50号3个白榆无性系的抗性较强。通过主成分分析,将12个白榆无性系分为3类,也得出类似结论,同时还对分类依据与应用状况进行系统分析与说明,为白榆耐盐性评价系统提供较为可靠的评价方法。

A research of screening of salt-tolerance of 12 elm clones in vitro culture

The main focuses of this thesis are evaluate and screening of the growth of 12 Elm Clones under different concentration of NaCl (0.3%,0.4%,0.5%,0.6%,0.7%,0.8%) in Vitro Culture. The impact of changes in the external environment and conditions on clone salt-tolerance of Ulmus pumila was excluded. By using the method of membership function, the ability of NaCl tolerance for the 12 elm clones has analyzed and evaluated. The result shows that No.58, No.30 and No.50 elm clones have strong resistance, also the result from principal component analysis are similarity; at the same time, the result of principal component analysis (PCA) divides the 12 Elm Clones into 3 classes according to the application situation, which provide a reliable evaluation methodology for the tolerance of elm clones toward NaCl.